Anthocyanin mutants of Japanese and common morning glories exhibit normal proanthocyanidin accumulation in seed coats

• Park Kyeung Il

  Department of Horticulture & Life Science, College of Life and Applied Sciences, Yeungnam University

• Nitasaka Eiji

  Department of Biological Science, Faculty of Science, Kyushu University

• Hoshino Atsushi

  National Institute for Basic Biology Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies)

抄録

Anthocyanin and proanthocyanidin biosynthesis pathways are believed to overlap. This study examined proanthocyanidin accumulation in seed coats of morning glories (Ipomoea nil and I. purpurea) carrying mutations in CHS-D, CHI, and ANS genes encoding chalcone synthase, chalcone isomerase, and anthocyanidin synthase, respectively. Chemical staining revealed that mutants accumulate proanthocyanidin normally. Thus, the tested genes are not essential to proanthocyanidin biosynthesis, but are essential to anthocyanin biosynthesis in flowers and stems. Based on the results and the I. nil draft genome sequence, the genes involved in proanthocyanidin biosynthesis, including a new copy of the flavanone 3-hydroxylase gene could be predicted. Moreover, the genome has no homologs for known enzymes involved in producing flavan-3-ols, the starter and extension units of proanthocyanidin. These results suggested that I. nil produces flavan-3-ols through an undiscovered biosynthesis pathway. To characterize proanthocyanidin pigmentation further, we conducted mutant screening using a large I. nil population. We discovered that the brown mutant lines (exhibiting brown seeds and normal anthocyanin pigmentation) do not accumulate proanthocyanidin in their seed coats. Thus, the brown mutation should be useful for further investigations into the various mechanisms controlling anthocyanin and proanthocyanidin pathways.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 35 (3), 259-266, 2018-09-25

  日本植物バイオテクノロジー学会

キーワード

• Anthocyanin
• <i>Ipomoea nil</i>
• <i>Ipomoea purpurea</i>
• proanthocyanidin
• seed coat

詳細情報

• CRID: 1390845713006388992
• NII論文ID: 130007495560
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.18.0613a
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 029264945
• Web Site: http://id.ndl.go.jp/bib/029264945
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029264945
• Web Site: https://www.jstage.jst.go.jp/article/plantbiotechnology/35/3/35_18.0613a/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可