Effect of wild grape on the signaling of histamine H₁ receptor gene expression responsible for the pathogenesis of allergic rhinitis

• Islam Rezwanul

  Department of Molecular Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School

• Mizuguchi Hiroyuki

  Laboratory of Pharmacology Faculty of Pharmacy Osaka Ohtani University

• Shaha Aurpita

  Department of Molecular Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School

• Nishida Kohei

  Department of Molecular Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School

• Yabumoto Masami

  Medical Corporation Kinshukai

• Ikeda Hisashi

  Nab co., ltd.

• Fujino Hiromichi

  Department of Molecular Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School

• Kitamura Yoshiaki

  Department of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School

• Fukui Hiroyuki

  Department of Molecular Studies for Incurable Diseases, Institute of Biomedical Sciences, Tokushima University Graduate School

• Takeda Noriaki

  Department of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School

Bibliographic Information

Other Title

• Effect of wild grape on the signaling of histamine H1 receptor gene expression responsible for the pathogenesis of allergic rhinitis

Abstract

As expression level of allergic disease‐sensitive genes are correlated with allergic symptom severity, suppression of these gene expressions could be good therapeutics. We have demonstrated that PKCδ signaling and NFAT signaling, involve in histamine H₁ receptor (H1R) and IL‐9 gene expressions, respectively, are responsible for the pathogenesis of allergic rhinitis. We explore anti‐allergic compounds that suppress these signaling pathways and found that wild grape (WG) contains such compounds. Here, we investigated the effect of WG hot water extract (WGE) on the signaling pathways for PKCδ‐mediated H1R and NFAT‐mediated IL‐9 gene expressions. WGE suppressed histamine/PMA‐induced H1R gene up‐regulation in HeLa cells. Toluene‐2,4‐diisocyanate (TDI)‐induced H1R mRNA elevation in TDI‐sensitized rats was also suppressed by WGE treatment. Treatment with WGE in combination with Awa‐tea, suppresses NFAT signaling‐mediated IL‐9 gene, markedly alleviated nasal symptoms. Furthermore, WGE suppressed PMA‐induced IL‐33 gene up‐regulation in Swiss 3T3 cells. Data suggest that combination of WGE, suppresses PKCδ signaling with Awa‐tea, suppresses NFAT signaling would have distinct clinical and therapeutic advantages as a substitute for anti‐allergic drugs. In addition, as the expression level of IL‐33 mRNA was correlated with the blood eosinophils number in patients with pollinosis, WG could alleviate eosinophilic inflammation through the suppression of IL‐33 gene expression. J. Med. Invest. 65:242‐250, August, 2018

Journal

• The Journal of Medical Investigation

  The Journal of Medical Investigation 65 (3.4), 242-250, 2018

  The University of Tokushima Faculty of Medicine

Keywords

• Allergic rhinitis
• histamine H₁ receptor
• Interleukin‐33
• nuclear factor of activated T‐cells
• protein kinase Cδ

Details

• CRID: 1390282763071555840
• NII Article ID: 130007495826
• NII Book ID: AA12022913
• DOI: 10.2152/jmi.65.242
• ISSN: 13496867
• ISSN: 13431420
• PubMed: 30282868
• Web Site: https://repo.lib.tokushima-u.ac.jp/112246
• Web Site: https://www.jstage.jst.go.jp/article/jmi/65/3.4/65_242/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2020012954
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed