Development of transgenic male-sterile rice by using anther-specific promoters identified by comprehensive screening of the gene expression profile database ‘RiceXPro’
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- Akasaka Maiko
- Institute of Crop Science, NARO Present address: Tohoku Agricultural Research Center, NARO
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- Taniguchi Yojiro
- Institute of Agrobiological Sciences, NARO
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- Oshima Masao
- Institute of Agrobiological Sciences, NARO Present address: Tsukuba-Plant Innovation Research Center, University of Tsukuba
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- Abe Kiyomi
- Institute of Agrobiological Sciences, NARO Present address: Biotherapy Institute of Japan Inc.
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- Tabei Yutaka
- Institute of Agrobiological Sciences, NARO
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- Tanaka Junichi
- Institute of Crop Science, NARO Graduate School of Life and Environmental Science, University of Tsukuba
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<p>Because genomic selection is designed for the population breeding of allogamous species, a successive outcrossing system is required for efficient use of genomic selection in autogamous crops, such as Oryza sativa L. (rice). Transgenic and dominant male-sterility is a suitable tool for efficient outcrossing of autogamous crops. Though there have been some reports of dominant male-sterile rice developed using transgenic technology, the flowering habit was substandard. Here, to isolate promoters that, when linked to a lethal gene, induce dominant male-sterility while retaining a good flowering habit, we identified 38 candidate genes with anther-specific expression by using the ‘RiceXPro’ database. We then evaluated the abilities of the near-upstream regions of these genes to induce male-sterility when linked to the lethal gene barnase and introduced into the rice cultivar ‘Nipponbare’. Seven of the 38 promoters induced clear dominant male-sterility; promoters expressed in the later stage of anther development induced male-sterility while retaining better flowering habits when compared to ones expressed in the early stage. These seven promoters could potentially be used to facilitate development of an efficient outcross-based breeding system in rice.</p>
収録刊行物
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- Breeding Science
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Breeding Science 68 (4), 420-431, 2018
日本育種学会
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詳細情報 詳細情報について
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- CRID
- 1390845713012026112
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- NII論文ID
- 130007496838
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- NII書誌ID
- AA11353132
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- ISSN
- 13473735
- 13447610
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- HANDLE
- 2241/00154595
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- NDL書誌ID
- 029294274
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可