Reactive Oxygen Species Production and Stimulated Endocytosis in Tobacco BY-2 Cells Treated with <i>Erwinia carotovora</i> Culture Filtrate

• Hirakawa Yumi

  Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo

• Hasezawa Seiichiro

  Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo

• Higaki Takumi

  International Research Organization for Advanced Science and Technology, Kumamoto University

Abstract

We previously established an experimental system for efficient pathogenic signal induced-cell death using tobacco BY-2 cells and culture filtrate of a plant pathogenic bacterium, Erwinia carotovora. Using this experimental system, cytoskeletal and vacuolar changes during the process of filtrate-induced cell death were characterized in detail. However, the initial events induced by the filtrate were largely unknown. In this study, reactive oxygen species (ROS) production and endocytosis stimulation were examined in BY-2 cells treated with the filtrate of E. carotovora. Transient ROS production within 2 min was observed after the filtrate treatment. Treatment with the Ca²⁺ chelator BAPTA or the protein kinase inhibitor K252a significantly inhibited ROS production in a dose-dependent manner, suggesting that ROS production depends on Ca²⁺ influx or protein phosphorylation. In addition, internalization of the endocytic marker FM4-64 was promoted by filtrate treatment within 30 min, suggesting that the filtrate treatment stimulated endocytosis. These results showed that ROS production and subsequent endocytosis stimulation are the initial events induced by culture filtrate of E. carotovora in BY-2 cells.

Journal

• CYTOLOGIA

  CYTOLOGIA 83 (3), 289-293, 2018-09-25

  Japan Mendel Society, International Society of Cytology

Keywords

• Endocytosis
• <i>Erwinia carotovora</i>
• Reactive oxygen species
• Tobacco BY-2 cell

Details

• CRID: 1390001288082243968
• NII Article ID: 130007497289
• DOI: 10.1508/cytologia.83.289
• ISSN: 13487019
• ISSN: 00114545
• Web Site: https://www.jstage.jst.go.jp/article/cytologia/83/3/83_D-18-00005/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed