Immunohistochemical Study of the Laminin α5 Chain and Its Specific Receptor, Basal Cell Adhesion Molecule (BCAM), in both Fetal and Adult Rat Pituitary Glands

• Azuma Morio

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Tsukada Takehiro

  Department of Biomolecular Science, Faculty of Science, Toho University

• Inagaki Takeshi

  Division of Forensic Medicine, Department of Anatomy, Jichi Medical University School of Medicine

• Casmad Fujianti

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Jindatip Depicha

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine Department of Anatomy, Faculty of Medicine, Chulalongkorn University

• Tofrizal Alimuddin

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Maliza Rita

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Batchuluun Khongorzul

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Syaidah Rahimi

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Ohno Nobuhiko

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Fujiwara Ken

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Kikuchi Motoshi

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine Laboratory of Natural History, Jichi Medical University School of Medicine

• Yashiro Takashi

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

抄録

Laminin, a major basement membrane protein, comprises three subunit chains: α, β, and γ chains. Among these chains, only the laminin α chain is capable of signaling via laminin receptors. Although laminin isoforms containing the α5 chain were reported to be the first laminin produced during rat anterior pituitary gland development, the functions of these isoforms are unknown. We used immunohistochemical techniques to localize the laminin α5 chain and its specific receptor, basal cell adhesion molecule (BCAM), in fetal and adult pituitary gland. Laminin α5 chain immunoreactivity was observed in the basement membrane of the primordial adenohypophysis at embryonic days 12.5 to 19.5. Double immunostaining showed that BCAM was present and co-localized with the laminin α5 chain in the tissue. Quantitative analysis showed that the laminin α5 chain and BCAM were expressed in the anterior pituitary gland during postnatal development and in adulthood (postnatal day 60). In the adult gland, co-localization of the laminin α5 chain and BCAM was observed, and BCAM was detected in both the folliculo-stellate cells and endothelial cells. These results suggest that laminin α5 chain signaling via BCAM occurs in both the fetal adenohypophysis and adult anterior pituitary gland.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 51 (5), 145-152, 2018-10-31

  日本組織細胞化学会

キーワード

• laminin
• basal cell adhesion molecule
• laminin receptor
• pituitary development
• immunohistochemistry

詳細情報

• CRID: 1390001288084719872
• NII論文ID: 130007502374
• DOI: 10.1267/ahc.18014
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/51/5/51_18014/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2020107704
• 本文言語コード: en
• データソース種別:
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