Identification of reference genes for quantitative PCR analyses in developing mouse gonads

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  • YOKOYAMA Toshifumi
    Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
  • OMOTEHARA Takuya
    Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan Department of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku, Tokyo 160-8402, Japan
  • HIRANO Tetsushi
    Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan Division of Drug and Structural Research, Life Science Research Center, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan
  • KUBOTA Naoto
    Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
  • YANAI Shogo
    Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
  • HASEGAWA Chinatsu
    Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
  • TAKADA Tadashi
    Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
  • MANTANI Yohei
    Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
  • HOSHI Nobuhiko
    Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan

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Abstract

<p>Stable reference genes are important for gene expression analyses such as quantitative PCR. The stability of 15 candidate reference genes that can be used to developing mouse gonads was thoroughly verified using combinations of multiple algorithms. The expression of these genes fluctuated greatly depending on the analysis period and/or gender. Peptidylprolyl isomerase A (Ppia) and polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) were the reference genes that were used stably for a wide analysis period in developing mouse gonads. Furthermore, the stable reference genes corresponding to the analysis period and/or gender were ranked. These results are useful for the selection of the optimal reference gene required for high-precision measurements.</p>

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