子宮頸部扁平上皮病変における HPV (human papillomavirus) 感染と細胞像の関連性  [in Japanese] Cytological findings associated with human papillomavirus infection in uterine cervical squamous lesions  [in Japanese]

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Author(s)

    • 下代 清香 SHIMODAI Sayaka
    • 九州大学大学院医学系学府保健学専攻|飯塚病院中央検査部|日本大学医学部病態病理学系人体病理学分野 Department of Health Sciences, Graduate School of Medical Sciences, Kyushu University|Department of Clinical Laboratory, Iizuka Hospital|Department of Human Pathology, Faculty of Medicine Pathophysiology, Nihon University
    • 加来 恒壽 KAKU Tsunehisa
    • 九州大学大学院医学研究院保健学部門 Department of Health Sciences, Faculty of Medical Sciences, Kyushu University
    • 杉島 節夫 SUGISHIMA Setsuo
    • 九州大学大学院医学研究院保健学部門 Department of Health Sciences, Faculty of Medical Sciences, Kyushu University

Abstract

<p><b>目的</b> : HR-HPV 感染に特徴的な細胞像の確立を目的とした.</p><p><b>方法</b> : 対象は HR-HPV 検査を併用した子宮頸部細胞診で ASC-US または LSIL と診断された, HR-HPV (+) 30 例, HR-HPV (−) 24 例の計 54 例とした. 標本中の多核細胞の核圧排像と koilocyte をカウントし, 画像解析ソフトを用いて核クロマチンの染色性と分布を評価した.</p><p><b>成績</b> : 二核細胞核圧排型は, HR-HPV (+) 群で有意に多くみられた. 二核細胞非核圧排型, 多核細胞, koilocyte の出現数に有意差はみられなかった. 核クロマチンについて, 染色性に有意差はみられなかったが, HR-HPV (+) 群で核辺縁が濃染する傾向がみられた.</p><p><b>結論</b> : HR-HPV 感染と, 1) 核圧排型二核細胞の出現, 2) RD 値における核クロマチン辺縁型の出現との関連性が示唆された.</p>

<p><i><b>Objective</b></i> : We sought to determine the characteristic cytological findings associated with HR-HPV infection in uterine cervical squamous lesions.</p><p><i><b>Study Design</b></i> : We examined 54 cervical smears obtained from 54 patients : the HR-HPV (+) group (n=30) and the HR-HPV (−) group (n=24). We counted the number of compression-positive multinucleated cells and number of koilocytes, and evaluated the stainability of nuclear chromatin and calculated the RD values using a digital imaging analysis software.</p><p><i><b>Results</b></i> : A significantly larger number of binucleated cells with compression was observed in the HR-HPV (+) group as compared to the HR-HPV (−) group. No significant inter group difference was observed in the numbers of binucleated cells without compression, multinucleated cells, or koilocytes. Regarding nuclear chromatin, although no significant inter group difference was observed in the stainability, the highest staining intensity tended to be localized at the nuclear periphery in the HR-HPV (+) group.</p><p><i><b>Conclusion</b></i> : Our results indicated an association between HR-HPV infection and (1) the appearance of binucleated cells with compression and (2) localization of the nuclear chromatin at the nuclear periphery, as shown by the RD values.</p>

Journal

  • The Journal of the Japanese Society of Clinical Cytology

    The Journal of the Japanese Society of Clinical Cytology 57(5), 251-258, 2018

    The Japanese Society of Clinical Cytology

Codes

  • NII Article ID (NAID)
    130007506203
  • Text Lang
    JPN
  • ISSN
    0387-1193
  • Data Source
    J-STAGE 
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