Spoilage Marker Metabolites and Pathway Analysis in Chilled Tan Sheep Meat Based on GC-MS

• You Liqin

  School of Agriculture, Ningxia University Food Science Research Institute of Ningxia University

• Guo Yansheng

  School of Agriculture, Ningxia University

• Luo Ruiming

  School of Agriculture, Ningxia University Food Science Research Institute of Ningxia University

• Fan Yanli

  School of Agriculture, Ningxia University Food Science Research Institute of Ningxia University

• Zhang Tonggang

  School of Agriculture, Ningxia University Food Science Research Institute of Ningxia University

• Hu Qianqian

  School of Agriculture, Ningxia University Food Science Research Institute of Ningxia University

• Bo Shuang

  School of Agriculture, Ningxia University Food Science Research Institute of Ningxia University

抄録

Metabolic changes of Tan sheep meat maintained at 0 °C for up to 8 d were identified based on gas chromatography time-of-flight mass spectrometry. Total viable counts (TVC), total volatile basic nitrogen (TVB-N) and pH were utilized as freshness indicators during meat storage. A relationship between meat freshness, metabolite accumulation and key metabolic pathways was postulated. Twenty-seven statistically significant metabolites were characterized. D-glyceric acid, phenylalanine, methionine, glucose-1-phosphate, D-(glycerol-phosphate), lysine, ribitol, asparagine and isomaltose were significantly (p < 0.01) correlated to the freshness indicators. A correlation coefficient with gluconic acid, citric acid and trans-4-hydroxy-L-proline and the freshness indicators ranged from 0.539 to 0.911. 1, 5-anhydroglucitol was significantly correlated with pH (p < 0.05) and TVC (p < 0.01). These metabolites were identified as potential spoilage biomarkers. Three major metabolic processes occurred during storage: metabolism of alanine, aspartate, and glutamate; an operational TCA cycle and metabolism of amino sugar and nucleotide sugar.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 24 (4), 635-651, 2018

  公益社団法人 日本食品科学工学会

キーワード

• metabolites profiling
• sheep meat
• spoilage
• biomarker
• pathways

詳細情報

• CRID: 1390845713023875968
• NII論文ID: 130007511538
• DOI: 10.3136/fstr.24.635
• ISSN: 18813984
• ISSN: 13446606
• Web Site: https://www.jstage.jst.go.jp/article/fstr/24/4/24_635/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可