Field Test of On-Site Treatment of 1,4-Dioxane-Contaminated Groundwater Using <i>Pseudonocardia</i> sp. D17

• Yamamoto Norifumi

  Technology Center, Taisei Corporation, Kanagawa, Japan

• Inoue Daisuke

  Division of Sustainable Energy and Environmental Engineering, Osaka University, Osaka, Japan

• Sei Kazunari

  Department of Health Science, Kitasato University, Kanagawa, Japan

• Saito Yuji

  Technology Center, Taisei Corporation, Kanagawa, Japan

• Ike Michihiko

  Division of Sustainable Energy and Environmental Engineering, Osaka University, Osaka, Japan

Bibliographic Information

Other Title

• Field test of on-site treatment of 1,4-dioxane-contaminated groundwater using Pseudonocardia sp. D17

Abstract

Contamination of groundwater by 1,4-dioxane has been detected in many regions worldwide, especially in illegal industrial waste dumping sites. As 1,4-dioxane is a potential human carcinogen that is highly resistant to most conventional biological and physicochemical treatments, the development of a practical application method for treating 1,4-dioxane-contaminated groundwater is strongly desirable. Therefore, this study aimed to develop an on-site treatment system for 1,4-dioxane-contaminated groundwater using Pseudonocardia sp. D17, and assess the feasibility of the developed system for long-term treatment. This bacterial strain is capable of constitutively degrading 1,4-dioxane and using it as a source of carbon and energy. The on-site treatment of 1,4-dioxane-contaminated groundwater was performed in a 450-L continuous-flow bioreactor using strain D17 with a hydraulic retention time of 12 ‒ 18 h. To maintain a sufficiently high microbial concentration, strain D17 was monitored by qPCR, targeting the thmC gene associated with 1,4-dioxane degradation, and inoculated when needed. 1,4-Dioxane removal to meet the Japanese environmental quality standard for groundwater pollution (0.05 mg/L) was achieved for nearly 3 months by the additional inoculation of strain D17. These results suggest that on-site treatment using strain D17 could provide a practical solution for the treatment of 1,4-dioxane-contaminated groundwater.

Journal

• Journal of Water and Environment Technology

  Journal of Water and Environment Technology 16 (6), 256-268, 2018

  Japan Society on Water Environment

Keywords

• 1
• 4-dioxane
• groundwater contamination
• bioremediation
• on-site treatment
• <i>Pseudonocardia</i> sp. D17.

Details

• CRID: 1390282763074985088
• NII Article ID: 130007528633
• DOI: 10.2965/jwet.18-033
• ISSN: 13482165
• Web Site: https://www.jstage.jst.go.jp/article/jwet/16/6/16_18-033/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed