Crystallographic analysis of the overlapping dinucleosome as a novel chromatin unit

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Author(s)

    • Nishimura Masahiro
    • Laboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo|Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University
    • Nozawa Kayo
    • Laboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo|Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University
    • Kurumizaka Hitoshi
    • Laboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo|Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University

Abstract

<p>Recent evidence has suggested that chromatin is not simply repeats of the canonical nucleosome, called the "octasome", but may include diverse repertoires of basic structural units. During the transcription process, a nucleosome is repositioned by a chromatin remodeler and collides with a neighboring nucleosome, thus creating an unusual nucleosome substructure termed the "overlapping dinucleosome". We previously developed a method for the large-scale preparation of the overlapping dinucleosome. This method enabled us to solve the crystal structure of the overlapping dinucleosome, which revealed an unexpected structure composed of an octameric histone core associated with a hexameric histone core lacking one H2A-H2B dimer.</p>

Journal

  • Biophysics and Physicobiology

    Biophysics and Physicobiology 15(0), 251-254, 2018

    The Biophysical Society of Japan

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