L-Type Calcium Channel-Mediated Zinc Wave Is Involved in the Regulation of IL-6 by Stimulating Non-IgE with LPS and IL-33 in Mast Cells and Dendritic Cells

• Uchida Ryota

  Laboratory of Immune Regulation, Graduate School of Pharmaceutical Sciences, Suzuka University of Medical Science

• Xiang Huihui

  Division of Functional Immunology, Institute for Genetic Medicine, Hokkaido University

• Arai Hiroya

  Laboratory of Immune Regulation, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science

• Kitamura Hidemitsu

  Division of Functional Immunology, Institute for Genetic Medicine, Hokkaido University

• Nishida Keigo

  Laboratory of Immune Regulation, Graduate School of Pharmaceutical Sciences, Suzuka University of Medical Science Laboratory of Immune Regulation, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science

抄録

The trace element zinc is essential for the immune system, and its dysregulation and deficiency results in impaired immune function. Recent studies have shown that zinc can behave as an intracellular signaling molecule in immune cells. We have previously demonstrated that L-type calcium channel (LTCC) is involved in the regulation of zinc signaling, Zinc wave and cytokine production by stimulating Fc epsilon receptor for immunoglobulin E (IgE) in mast cells. However, it is not known whether LTCC-mediated Zinc wave is required for cytokine production by stimulation of toll-like receptors and cytokine receptors in mast cells. Here we report that stimulation of toll-like receptors and cytokine receptors can induce Zinc wave in mast cells and regulate the expression of cytokine genes. The LTCC antagonist nicardipine inhibited lipopolysaccharide (LPS)- and interleukin-33 (IL-33)-mediated Zinc wave and the induction of cytokine genes such as IL-6. Consistent with these results, the zinc chelator N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) also inhibited LPS- and IL-33-induced cytokine gene expression. Furthermore, LPS induced Zinc wave not only in mast cells but also in dendritic cells. Together, these observations show that Zinc wave is activated by various stimuli and is linked to cytokine gene induction in immune cells.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 42 (1), 87-93, 2019-01-01

  公益社団法人 日本薬学会

キーワード

• zinc signaling
• mast cell
• dendritic cell
• lipopolysaccharide (LPS)
• cytokine
• nuclear factor-kappaB (NF-κB)

詳細情報

• CRID: 1390564238060402432
• NII論文ID: 130007542207
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b18-00565
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 029410923
• PubMed: 30416166
• Web Site: http://id.ndl.go.jp/bib/029410923
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029410923
• Web Site: https://www.jstage.jst.go.jp/article/bpb/42/1/42_b18-00565/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2019253770
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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