Induction of Mucosal Humoral Immunity by Subcutaneous Injection of an Oil-emulsion Vaccine against <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Enteritidis in Chickens

• Ishida Yuuichi

  Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan

• Sakai Eishi

  Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan

• Sato Katsuo

  Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan

• Sugiyama Einori

  Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan

• Mima Kazuyuki

  Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan

• Taneno Akira

  Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan

• Shimomura Hirofumi

  Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan Department of Nutritional Science, Faculty of Human Life Science, Shokei University, 2-6-78, Kuhonji, Chuo-ku, Kumamoto-shi, Kumamoto 862-8678, Japan

• Cui Longzhu

  Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan

• Hirai Yoshikazu

  Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan Tamano Institute of Health and Human Services, 1-1-20 Chikko Tamano-shi, Okayama 706-0002, Japan

Abstract

Salmonella enterica subsp. enterica serovar Enteritidis (SE) is one of the major causes of food poisoning. Much effort has been made to develop a vaccine for the prevention of SE colonization and infection in poultry. However, the effect of inactivated whole-cell SE vaccines on the bacterial attachment has not been clarified. This study investigated the immune responses to a killed whole-cell SE vaccine in chickens and the effect of vaccination on the bacterial attachment of SE to cultured Vero cells. A 1 ml dose of 10⁸–10⁹ CFU viable SE bacterial cells was orally administered to chickens at 4 weeks or 10 months post vaccination. The number (CFU) of SE in 1 g of cecal droppings was counted on day 6 after administration. The SE CFUs were significantly lower (p < 0.05) in the vaccinated chickens, not only at 4 weeks but also at 10 months after vaccination, than in the unvaccinated control chickens. Anti-SE IgG and anti-SE IgA were detected using enzyme-linked immunosorbent assay (ELISA) in serum and intestinal and oviduct fluid samples from vaccinated chickens. Adhesion of heat-killed SE cells to Vero cells was reduced by pre-treatment of the bacteria by the vaccinated chicken-derived intestinal fluid, indicating the potential of the vaccine-induced antibody to prevent SE adhesion to epithelial cell surfaces.

Journal

• Food Safety

  Food Safety 6 (4), 151-155, 2018

  Food Safety Commission, Cabinet Office, Government of Japan

Keywords

• bacterial attachment
• inactivated oil-emulsion SE vaccine
• mucosal humoral immunity
• Salmonella Enteritidis

Details

• CRID: 1390845713081364864
• NII Article ID: 130007546578
• DOI: 10.14252/foodsafetyfscj.2018003
• ISSN: 21878404
• Web Site: https://www.jstage.jst.go.jp/article/foodsafetyfscj/6/4/6_2018003/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2020165233
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed