Synthesis of a Peptide-Human Telomere DNA Conjugate as a Fluorometric Imaging Reagent for Biological Sodium Ion

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  • SATO Shinobu
    Research Center for Bio-microsensing Technology, Kyushu Institute of Technology Department of Applied Chemistry, Kyushu Institute of Technology
  • IMAICHI Yuuki
    Department of Applied Chemistry, Kyushu Institute of Technology
  • YOSHIURA Yukiko
    Graduate School of Environmental Engineering, The University of Kitakyuhsu
  • NAKAZAWA Koji
    Graduate School of Environmental Engineering, The University of Kitakyuhsu
  • TAKENAKA Shigeori
    Research Center for Bio-microsensing Technology, Kyushu Institute of Technology Department of Applied Chemistry, Kyushu Institute of Technology

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<p>A peptide-oligonucleotide conjugate (1) was synthesized by the attachment of FAM, TAMRA, and biotin moieties to a telomere DNA sequence of 5′-TAG GGT TAG GGT TAG GGT TAG GG-3′. This conjugate was induced to be an anti-parallel structure in the presence of sodium ion (Na+), whereas a hybrid one was formed under potassium ion (K+) as a monitoring by circular dichromic spectra. The conformation change of this conjugate gave an effective FRET signal change upon the addition of NaCl, compared with the case of KCl. Under 5 mM KCl as an extracellular condition, a FRET change was observed upon addition of NaCl and quantitative FRET change was observed in 0 – 250 mM NaCl. This conjugate was immobilized on the cell surface through a sugar chain on the cell, biotinyl concanavallin A and streptavidin. This conjugate was utilized for Na+ sensing based on anti-parallel tetraplex formation with Na+.</p>

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  • Analytical Sciences

    Analytical Sciences 35 (1), 85-90, 2019-01-10

    社団法人 日本分析化学会

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