新規の選択培地であるVi GBS寒天培地による腟および肛門部からの<i>Streptococcus agalactiae</i>の検出性能  [in Japanese] Performance of a Novel Selective Agar Medium, Vi GBS Agar, for Detection of <i>Streptococcus agalactiae</i> from Vaginal and Anorectal Specimens  [in Japanese]

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Author(s)

    • 保坂 好恵 HOSAKA Yoshie
    • 順天堂大学医学部附属順天堂医院臨床検査部 Department of Clinical Laboratory, Juntendo University Hospital
    • 中村 文子 NAKAMURA Ayako
    • 順天堂大学医学部附属順天堂医院臨床検査部 Department of Clinical Laboratory, Juntendo University Hospital
    • 佐野 麻衣 SANO Mai
    • 順天堂大学医学部附属順天堂医院臨床検査部 Department of Clinical Laboratory, Juntendo University Hospital
    • 土屋 浩二 TSUCHIYA Koji
    • 順天堂大学医学部附属順天堂医院臨床検査部 Department of Clinical Laboratory, Juntendo University Hospital
    • 三澤 成毅 MISAWA Shigeki
    • 順天堂大学医学部附属順天堂医院臨床検査部 Department of Clinical Laboratory, Juntendo University Hospital
    • 堀井 隆 HORII Takashi
    • 順天堂大学医学部附属順天堂医院臨床検査部 Department of Clinical Laboratory, Juntendo University Hospital
    • 大坂 顯通 OHSAKA Akimichi
    • 順天堂大学医学部附属順天堂医院臨床検査部|順天堂大学大学院医学研究科輸血・幹細胞制御学 Department of Clinical Laboratory, Juntendo University Hospital|Department of Transfusion Medicine and Stem Cell Regulation, Juntendo University School of Medicine

Abstract

<p><i>Streptococcus agalactiae</i>の選択分離培地であるVi GBS寒天培地による妊婦の腟および肛門部からの検出性能を評価した。<i>S. agalactiae</i>臨床分離株98株中97株は,Vi GBS上に良好に発育し,培養24時間以内に特徴的な赤紫色の集落を形成した。腟または肛門内の常在菌はほとんどVi GBSに発育しなかった。<i>Enterococcus</i> spp.,<i>Streptococcus mitis</i>,<i>Pseudomonas aeruginosa</i>,および<i>Candida</i> spp.はVi GBSに発育したが,集落が着色しない,または極めて小さな集落であり,<i>S. agalactiae</i>と容易に区別できた。綿棒で採取された腟分泌物287検体は従来法であるヒツジ血液寒天/BTB乳糖寒天分画培地,Vi GBSの順に塗布し,35℃,24時間,好気培養した。これらのうち,従来法との一致率は98.6%(283/287)であった。不一致は4検体認められ,2検体は検体中の菌量が極めて少ない検体であった。残る2検体はVi GBSで検出されたが,BA/BTBでは検出されなかった。糞便検体に<i>S. agalactiae</i>を人工的に混合した模擬検体は,<i>Enterococcus</i> spp.による発育抑制は認められず,全6検体から<i>S. agalactiae</i>を回収できた。Vi GBS寒天培地を用いた直接平板培養は,高い選択性能を有し,妊婦からの<i>S. agalactiae</i>の分離に有用と考えられた。</p>

<p>We evaluated the performance of a commercially prepared selective medium, Vi GBS agar, for the screening of <i>Streptococcus agalactiae</i> from vaginal and anorectal specimens from pregnant women. Ninety-seven out of 98 clinical isolates of <i>S. agalactiae</i> grew well on Vi GBS agar and exhibited characteristic red-purple colonies within 24 h of incubation. Few microorganisms that comprise the normal flora of the vagina and rectum grew on Vi GBS agar. Some strains of <i>Enterococcus</i> spp., <i>Streptococcus mitis</i>, <i>Pseudomonas aeruginosa</i>, and <i>Candida</i> spp. grew on Vi GBS agar; however, these colonies were nonpigmented or very small, and could be clearly distinguished from those of <i>S. agalactiae</i>. A total of 287 swab specimens from the vagina and anorectum were directly plated onto Vi GBS subsequent to growth on sheep blood agar/BTB lactose agar biplate media (BA/BTB), and then incubated overnight at 35ºC. Of these, 98.6% (283/287) of the isolates were <i>S. agalactiae</i> and matched those of the conventional culture. Out of the four discrepant results, two false negatives were from specimens with low bacterial counts, and the remaining two were successfully isolated on Vi GBS agar, but not on BA/BTB. Vi GBS agar recovered <i>S. agalactiae</i> from six stool specimens that were artificially mixed, without the inhibition of its growth by <i>Enterococcus</i> from the stool. Our study suggests that direct plating onto Vi GBS agar shows high performance for the screening of <i>S. agalactiae</i> colonization in pregnant women.</p>

Journal

  • Japanese Journal of Medical Technology

    Japanese Journal of Medical Technology 68(1), 49-55, 2019

    Japanese Association of Medical Technologists

Codes

  • NII Article ID (NAID)
    130007557507
  • Text Lang
    JPN
  • ISSN
    0915-8669
  • Data Source
    J-STAGE 
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