Methylotrophic recombinant <i>Pichla pastoris</i> for phytase production on D-mannitol/methanol optimization

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Author(s)

    • Bernard Ndayambaje Jean
    • Department of Chemistry, College of Science and Technology, University of Rwanda KN 7 Ave, Kigali, P.O. Box 3900 Kigali Rwanda
    • Janvier Habinshuti
    • Department of Chemistry, College of Science and Technology, University of Rwanda KN 7 Ave, Kigali, P.O. Box 3900 Kigali Rwanda
    • Angelique Ingabire
    • Dpartment of microbial analysis, laboratory, Rwanda Agriculture board KK 18 Ave, Kigali, P. O Box 5016 Kicukiro District, Rubilizi Kigali-Rwanda
    • Emmerence Nteziyaremye Beneyo
    • Department of Chemistry, College of Science and Technology, University of Rwanda KN 7 Ave, Kigali, P.O. Box 3900 Kigali Rwanda
    • Gratien Habarurema
    • Department of Chemistry, College of Science and Technology, University of Rwanda KN 7 Ave, Kigali, P.O. Box 3900 Kigali Rwanda
    • Meenakshisundaram
    • Department of Biotechnology, Anna University Chennai, Tamil Nadu, 600025, India

Abstract

<p>D-mannitol addition as a co-substrate with methanol at the induction phase during fermentation by <i>Pichia pastoris</i> (Mut+) is a new beneficial technology for recombinant protein production. The major challenges as heat generation and high oxygen demand are increasing during induction phases with methanol at large scale and this causes high oxygen demand during cultivation time. One way possible to reduce the oxygen demand for getting more protein productivity is the addition of D-mannitol along with methanol during induction time. The co-feeding strategy was optimized to produce phytase activity of 10280 U/ml which is two and half time higher compare to methanol fed alone and we suggest that it could be used as food additives for non-ruminant animals. A deep understanding of regulation of <i>AOX1</i> promoter is that, the physiology of the cells which are being used to govern protein production during methanol/D-mannitol strategy is still not developed at large.</p>

Journal

  • Bulletin of the Institute of Tropical Agriculture, Kyushu University

    Bulletin of the Institute of Tropical Agriculture, Kyushu University 41(0), 97-105, 2018

    Institute of Tropical Agriculture, Kyushu University

Codes

  • NII Article ID (NAID)
    130007558448
  • Text Lang
    ENG
  • ISSN
    0915-499X
  • Data Source
    J-STAGE 
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