質量分析による<i>Saccharomyces cerevisiae</i>菌株識別に対する低温処理の影響  [in Japanese] The Effect of Cold Incubation before Mass Spectrometry Identification on Different <i>Saccharomyces cerevisiae </i>Strains  [in Japanese]

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Author(s)

    • 半谷 朗 Hanya Akira
    • あいち産業科学技術総合センター食品工業技術センター Aichi Center for Industry and Science Technology

Abstract

<p>市販パン酵母および自然界から収集した野生の<i>S. cerevisiae</i>についてMALDI-TOF MSを用いてマススペクトルを収集し,類似したマススペクトルのパターンを有したパン酵母株と野生酵母株の組み合わせを複数選び出した.クラスター分析の結果,これらの菌株の組み合わせは互いのマススペクトルが酷似していた.しかし,コロニーが発生した寒天培地を冷蔵処理し,その後再びマススペクトルを収集してクラスター分析を行ったところ,パン酵母株と野生酵母株のマススペクトルは独立したクラスターを形成し,菌株の識別性が向上することが判明した.</p>

<p>This study was carried out to evaluate the effect of cold stress on matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) cluster analysis of <i>Saccharomyces cerevisiae</i>. Seven pair strains including one bread yeast strain and one or two wild type strains of <i>S. cerevisiae </i>were selected from yeast strain stocks at the Aichi Center for Industry and Science Technology. By using MALDI-TOF MS cluster analysis, those pair strains were shown as being not separated into single strain clusters. When those colonies were re-incubated at low temperatures before MALDI-TOF MS preparation, all pair strains were found to have separated into single strain clusters. These distinctions show that cold stress proteins could be used as biomarkers for MALDI-TOF MS identification of <i>S. cerevisiae </i>strains.</p>

Journal

  • Nippon Shokuhin Kagaku Kogaku Kaishi

    Nippon Shokuhin Kagaku Kogaku Kaishi 66(1), 18-26, 2019

    Japanese Society for Food Science and Technology

Codes

  • NII Article ID (NAID)
    130007575093
  • NII NACSIS-CAT ID (NCID)
    AN10467499
  • Text Lang
    JPN
  • ISSN
    1341-027X
  • NDL Article ID
    029429857
  • NDL Call No.
    Z17-126
  • Data Source
    NDL  J-STAGE 
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