Heat shock transcription factor σ³² defective in membrane transport can be suppressed by transposon insertion into genes encoding a restriction enzyme subunit or a putative autotransporter in Escherichia coli
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- Yura Takashi
- Faculty of Life Sciences, Kyoto Sangyo University
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- Miyazaki Ryoji
- Institute for Frontier Life and Medical Sciences, Kyoto University
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- Fujiwara Keigo
- Faculty of Life Sciences, Kyoto Sangyo University
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- Ito Koreaki
- Faculty of Life Sciences, Kyoto Sangyo University
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- Chiba Shinobu
- Faculty of Life Sciences, Kyoto Sangyo University
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- Mori Hiroyuki
- Institute for Frontier Life and Medical Sciences, Kyoto University
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- Akiyama Yoshinori
- Institute for Frontier Life and Medical Sciences, Kyoto University
書誌事項
- タイトル別名
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- Heat shock transcription factor σ<sup>32</sup> defective in membrane transport can be suppressed by transposon insertion into genes encoding a restriction enzyme subunit or a putative autotransporter in <i>Escherichia coli</i>
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抄録
<p>Heat shock transcription factor σ32 of Escherichia coli plays a major role in protein homeostasis and requires membrane localization for regulation. We here report that a strongly deregulated I54N-σ32 mutant defective in association with the membrane can be phenotypically suppressed by Tn5 insertion into the mcrC or ydbA2 gene, encoding a restriction enzyme subunit or part of a putative autotransporter, respectively. The suppression is specific for mutant I54N-σ32 and reduces its activity but not its abundance or stability. Moreover, the deregulated phenotype of I54N-σ32 is effectively suppressed by a plasmid carrying the same mcrC::Tn5 mutation. In contrast, deletion of the mcrC or ydbA2 gene hardly affects I54N-σ32 activity. These results, taken together, suggest that the truncated form of McrC (and presumably also of YdbA2) protein produced by the Tn5 insertion interacts specifically with I54N-σ32 to reduce its activity without substantially affecting its amount or stability.</p>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 93 (6), 229-235, 2018-12-01
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390564238062034432
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- NII論文ID
- 130007582071
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 029596504
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- PubMed
- 30531155
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可