GM-CSF Stimulates Mouse Macrophages and Causes Inflammatory Effects <i>in Vitro</i>
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- Suzuki Takuma
- Department of Periodontology, Tsurumi University School of Dental Medicine
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- Hayakawa Tohru
- Department of Dental Engineering, Tsurumi University School of Dental Medicine
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- Gomi Kazuhiro
- Department of Periodontology, Tsurumi University School of Dental Medicine
Bibliographic Information
- Other Title
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- GM-CSF Stimulates Mouse Macrophages and Causes Inflammatory Effects in Vitro
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Abstract
<p>Macrophages are involved at an early stage of the inflammatory reaction and have an important role in wound healing. Granulocyte-macrophage colony stimulating factor (GM-CSF) stimulates the proliferation and differentiation of macrophages. We investigated the secretion of tumor necrosis factor-α (TNF-α) and interleukin-4 (IL-4) from mouse macrophages (RAW264.7) activated by GM-CSF. RAW264.7 cells were cultured on titanium (Ti) discs. Secretion of TNF-α and IL-4 was evaluated using enzyme-linked immunosorbent assay (ELISA) at 24 h and 48 h. Cell morphologies were observed using SEM, and cell viability was accessed by an MTT assay.</p><p>GM-CSF caused rough and irregular surface morphology on the macrophages and resulted in a significant difference in cell viability after 48 h (p<0.05). TNF- α secretion significantly decreased after 48 h without GM-CSF compared with that at 24 h (p<0.05). GM-CSF significantly increased the secretion of TNF-α after 24 h and 48 h (p<0.05). IL-4 secretion was significantly different with or without GM-CSF stimulation at 24 h and 48 h (p<0.05). There was a significant increase in IL-4 secretion 24 h and 48 h after GM-CSF stimulation (p<0.05).</p><p>These results suggest that macrophage stimulated GM-CSF may promote secretion of anti-inflammatory and pro-inflammatory cytokines on Ti.</p>
Journal
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 28 (1), 37-42, 2019
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
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Details 詳細情報について
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- CRID
- 1390282763095602560
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- NII Article ID
- 130007585268
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- NII Book ID
- AA11074332
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- ISSN
- 1880828X
- 13417649
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- NDL BIB ID
- 029493792
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed