結晶調湿Ｘ線回折法と 室温測定の再評価

• 熊坂 崇

  高輝度光科学研究センタータンパク質結晶解析推進室

• 馬場 清喜

  高輝度光科学研究センタータンパク質結晶解析推進室

• 河村 高志

  高輝度光科学研究センタータンパク質結晶解析推進室

書誌事項

タイトル別名

• Recurrence of room-temperature measurement in macromolecular crystallography under humidity control

抄録

  Protein crystals are generally wet and fragile, thus easily suffer from dehydration and temperature change. To preserve the crystals from such sudden physicochemical conditional changes, we developed a crystal mounting method, humid air and glue coating (HAG) method. This method works well to maintain most protein crystals under both cryogenic and room temperature conditions. Room-temperature measurement has recently advanced in time-resolved analysis by serial femtosecond crystallography (SFX) method using X-ray free electron laser (XFEL) facilities, and emerges dynamical properties of proteins, leading to its recurrence even in synchrotron experiments. Here we show the brief guidance to the method and its application, and discuss the perspective of room-temperature structural analysis in macromolecular crystallography.

収録刊行物

• 日本結晶成長学会誌

  日本結晶成長学会誌 45 (4), n/a-, 2019

  日本結晶成長学会

詳細情報

• CRID: 1390845713050473472
• NII論文ID: 130007585417
• DOI: 10.19009/jjacg.3-45-4-02
• ISSN: 21878366
• ISSN: 03856275
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可