Investigation of the Mechanism for Rapid Antigen-Antibody Reaction Using Electric Field Mixing (EFM)

• NAKAMURA Ryuta

  秋田県産業技術センター

• KUSUMI Takayuki

  秋田県産業技術センター

• OKUBO Yoshinobu

  秋田県産業技術センター

• NANJO Hiroshi

  秋田大学医学部附属病院

• MINAMIYA Yoshihiro

  秋田大学大学院医学系研究科

• AKAGAMI Yoichi

  秋田県産業技術センター

Bibliographic Information

Other Title

• 電界撹拌技術を用いた抗原抗体反応の迅速メカニズムの解明

Abstract

By applied electric field mixing (EFM) showed antigen-antibody reaction time in an immunohistochemical staining can be shortened from 90 minutes of conventional method to 10 minutes. In this study, EFM examined the mechanism which makes antigen-antibody reaction of an immunohistochemical staining quicken. As a result, we obtained that it has the mechanism which a reaction time of an immunohistochemical staining is shorten because antigen and antibody improve to increase the frequencies of contact under EFM compared with conventional method. Further, we investigated the effect of AC electric field is applied on antibody. As a result, it found that by applied AC electric field to antibody liquid, antibody dispersed and stabilized, therefore, staining quality is improved.

Journal

• Journal of the Japan Society for Precision Engineering

  Journal of the Japan Society for Precision Engineering 85 (2), 208-212, 2019-02-05

  The Japan Society for Precision Engineering

Keywords

• AC electric field
• electric field mixing
• antigen-antibody reaction
• immunohistochemical staining
• Zeta potential

Details

• CRID: 1390282763097045504
• NII Article ID: 130007590595
• DOI: 10.2493/jjspe.85.208
• ISSN: 1882675X
• ISSN: 09120289
• Web Site: https://www.jstage.jst.go.jp/article/jjspe/85/2/85_208/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed