Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells
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- HASEGAWA Hiroki
- Laboratory of Reproductive Physiology, Graduate School of Agriculture, Tottori University, Tottori 680-8553, Japan
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- NISHIMURA Ryo
- Laboratory of Reproductive Physiology, Graduate School of Agriculture, Tottori University, Tottori 680-8553, Japan Laboratory of Theriogenology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
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- YAMASHITA Masamichi
- Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
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- YAMAGUCHI Takeshi
- Laboratory of Crop Husbandry, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
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- HISHINUMA Mitsugu
- Laboratory of Theriogenology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
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- OKUDA Kiyoshi
- Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
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抄録
<p> A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. To elucidate the mechanism involved in regulation of luteal cell function during CL development, we compared the effect of hypoxia on P4 production by cultured bovine early and mid luteal cells. Luteal cells obtained from early and mid CL were incubated under different O2 concentrations (20% and 3%) with or without hCG (1 U/ml) for 6 h and 24 h. After 6 h of culture in the presence of hCG, P4 production was not affected by hypoxia whereas decrease in its production by mid luteal cells was observed. After 24 h of culture, P4 production was significantly decreased by hypoxia in both stages of luteal cells regardless of the use of hCG. At 6 h of culture, hypoxia increased mRNA expression of hydroxyl-Δ-5-steroid dehydrogenase, 3β- and steroid Δ-isomerase 1 (HSD3B1) in early luteal cells, and decreased mRNA expression of cytochrome P450 cholesterol side chain cleavage (CYP11A1) enzyme in mid luteal cells. At 24 h of culture, mRNA expressions of steroidogenic acute regulatory protein (STAR), CYP11A1, and HSD3B1 were not affected by hypoxia in both stages of luteal cells. The overall results suggest that early luteal cells maintain P4 production under hypoxic conditions, and hypoxia-induced HSD3B1 may support this P4 production in the bovine early CL.</p>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 65 (1), 67-72, 2019
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390845713052189696
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- NII論文ID
- 130007591420
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- NII書誌ID
- AA12018734
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 029510938
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- PubMed
- 30487374
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
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- PubMed
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- 使用不可