小児の歯肉炎評価におけるGCFの応用に関する検討  [in Japanese] Application of GCF for Gingivitis Assessment in Children  [in Japanese]

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Author(s)

    • 角田 聡子 KAKUTA Satoko
    • 九州歯科大学地域健康開発歯学分野 Division of Community Oral Health Development, Kyushu Dental University
    • 玉木 直文 TAMAKI Naofumi
    • 徳島大学大学院医歯薬学研究部予防歯学分野 Department of Preventive Dentistry, Tokushima University Graduate School of Biomedical Sciences
    • 伊藤 博夫 ITO Hiro-O
    • 徳島大学大学院医歯薬学研究部予防歯学分野 Department of Preventive Dentistry, Tokushima University Graduate School of Biomedical Sciences
    • 邵 仁浩 SOH Inho
    • 九州歯科大学口腔保健学科 School of Oral Health Sciences, Kyushu Dental University
    • 葭原 明弘 YOSHIHARA Akihiro
    • 新潟大学大学院医歯学総合研究科口腔保健学分野 Division of Oral Science for Health Promotion, Department of Oral Health and Welfare, Niigata University
    • 福井 誠 FUKUI Makoto
    • 徳島大学大学院医歯薬学研究部予防歯学分野 Department of Preventive Dentistry, Tokushima University Graduate School of Biomedical Sciences
    • 諏訪間 加奈 SUWAMA Kana
    • 新潟大学大学院医歯学総合研究科口腔保健学分野 Division of Oral Science for Health Promotion, Department of Oral Health and Welfare, Niigata University
    • 鶴田 実穂 TSURUTA Miho
    • 鹿児島大学病院発達系歯科センター口腔保健科 Division of Preventive Dentistry, Kagoshima University Hospital
    • 平山 綾 HIRAYAMA Aya
    • 九州歯科大学地域健康開発歯学分野 Division of Community Oral Health Development, Kyushu Dental University
    • 片岡 正太 KATAOKA Syota
    • 九州歯科大学地域健康開発歯学分野 Division of Community Oral Health Development, Kyushu Dental University

Abstract

<p> 歯肉炎の評価は視診もしくはプロービングによる方法が一般的であり,検診の場で使用可能な非侵襲的で簡便性,客観性,再現性を併せもつ検査方法はないのが現状である.本研究では,歯肉溝滲出液( GCF) 中の成分が歯肉炎バイオマーカーとして有用であるかを検討した.</p><p> 対象は北九州市内某小学校5,6年生107名である.ブロッティングブラシ(以下,ブラシ)で採取したGCF中の<i>α</i>-1 アンチトリプシン(A1AT),ラクトフェリン(Lf) 他4成分(AST,ALT,ALP,LDH)を定量し,PMA index(PMA),プラーク付着状態(PlI)との比較を行った.PMA,PlIを低値群と高値群に2値化し,GCF成分との比較を行ったところ,PMAではすべての成分,PlIではLDH以外の成分で有意(<i>p</i><0.05)に関連していた.さらに,ROC曲線から求めたPMA,PlIに対する各成分のカットオフ値はそれぞれ,A1ATが283.8,248.3,Lfが152.1,114.4,ASTが4.5,2.5,ALTはともに1.5,ALPは2.5,PMAでのLDHが26.0であった.陽性尤度比は1.5から3.0程度となった.</p><p> 以上の結果からGCF中A1AT,Lf,AST,ALTおよびALPは小児を対象とした歯肉炎スクリーニングのバイオマーカーとして有用であることが示唆された.</p>

<p> Assessment of gingivitis is generally dependent on visual examination and probing. Currently, there is no procedure available that is non-invasive, easily performed, objective, and reproducible for the screening of gingivitis. In this study, we investigated whether substances in the gingival crevicular fluid (GCF) can be useful as biomarkers for gingivitis. The participants were 107 elementary school children (fifth and sixth graders) in Kitakyushu City. We collected samples of GCF from each child using a blotting brush and quantitated the levels of <i>α</i>-1 antitrypsin (A1AT), lactoferrin (Lf), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in the fluid. We then compared the results with oral gingival findings, such as the PMA index (PMA), community periodontal index (CPI), and plaque attachment index (PlI). The PMA and PlI results were binarized into a low-value group and a high-value group and then compared with each GCF substance. Significant differences (<i>p</i><0.05) were identified for all substances when compared with the PMA results and for all substances, except LDH, when compared with the PlI results. The cut-off points from the ROC curve analysis for these substances (A1AT, Lf, AST, ALT, ALP, and LDH) compared with the PMA results were 283.8, 152.1, 4.5, 1.5, 2.5, and 26.0, respectively, and those compared with the PlI results were 248.3, 114.4, 2.5, 1.5, and 2.5, respectively. The positive likelihood ratio was approximately 1.5 to 3.0.</p><p> These results suggest that A1AT, Lf, AST, ALT, and ALP levels in GCF may be useful as biomarkers for gingivitis screening in children.</p>

Journal

  • JOURNAL OF DENTAL HEALTH

    JOURNAL OF DENTAL HEALTH 69(1), 19-26, 2019

    Japanese Society for Oral Health

Codes

  • NII Article ID (NAID)
    130007592667
  • NII NACSIS-CAT ID (NCID)
    AN00081407
  • Text Lang
    JPN
  • ISSN
    0023-2831
  • NDL Article ID
    029499933
  • NDL Call No.
    Z19-144
  • Data Source
    NDL  J-STAGE 
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