Three Major Nucleotide Polymorphisms in the <i>Waxy</i> Gene Correlated with the Amounts of Extra-long Chains of Amylopectin in Rice Cultivars with S or L-type Amylopectin:Nucleotide Polymorphisms in <i>Waxy</i> Genes Responsible for Extra-long Chain Content in Rice

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Author(s)

    • Crofts Naoko Crofts Naoko
    • Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University
    • Itoh Ayaka Itoh Ayaka
    • Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University
    • Abe Misato Abe Misato
    • Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University
    • Miura Satoko Miura Satoko
    • Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University
    • Bao Jinsong Bao Jinsong
    • Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University
    • Fujita Naoko Fujita Naoko
    • Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University

Abstract

<p>Extra-long chains (ELC) of amylopectin in rice endosperm are synthesized by granule-bound starch synthase I encoded by the <i>Waxy </i>(<i>Wx</i>) gene, which primarily synthesizes amylose. Previous studies showed that single nucleotide polymorphisms (SNP) in intron 1 and exon 6 of the <i>Wx</i> gene influences ELC amount. However, whether these SNPs are conserved among rice cultivars and if any other SNPs are present in the <i>Wx</i> gene remained unknown. Here, we sequenced the <i>Wx</i> gene from 17 rice cultivars with S or L-type amylopectin, including those with known ELC content and those originating in China with unique starch properties, as well as typical japonica and indica cultivars. In addition to the two SNPs described above, an additional SNP correlating with ELC content was found in exon 10. Low ELC cultivars (<3.0 %) had thymine at the splicing donor site of intron 1, Tyr224 in exon 6, and Pro415 in exon 10. Cultivars with moderate ELC content (4.1–6.9 %) had guanine at the splicing donor site of intron 1, Ser224 in exon 6, and Pro415 in exon 10. Cultivars with high ELC content (7.7–13.9 %) had guanine at the splicing donor site of intron 1, Tyr224 in exon 6, and Ser415 in exon 10. The chain length distribution pattern of amylopectin was correlated with the amounts of SSIIa found in starch granules and gelatinization temperature, but not with ELC content. The combinations of SNPs in the <i>Wx</i> gene found in this study may provide useful information for screening specific cultivars with different ELC content.</p>

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 66(1), 37-46, 2019

    The Japanese Society of Applied Glycoscience

Codes

  • NII Article ID (NAID)
    130007602674
  • NII NACSIS-CAT ID (NCID)
    AA11809133
  • Text Lang
    ENG
  • ISSN
    1344-7882
  • NDL Article ID
    029567323
  • NDL Call No.
    Z78-A693
  • Data Source
    NDL  J-STAGE 
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