Immunohistochemical Mapping of Bcl9 Using Two Antibodies that Recognize Different Epitopes Is Useful to Characterize Juvenile Development of Hepatocellular Carcinoma in Myanmar

• Soe Myat Thu

  Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences

• Shibata Yasuaki

  Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences

• Win Htun Myo

  Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences

• Abe Kuniko

  Department of Pathology, Japanese Red Cross Nagasaki Atomic Bomb Hospital

• Soe Kyaw

  Department of Medical Research

• Win Than Nay

  Department of Hepatobiliary and Pancreatic Surgery, Yangon Specialty Hospital

• Lwin Thann

  Department of Hepatobiliary and Pancreatic Surgery, Yangon Specialty Hospital

• Phone Kyaw Myat

  Department of Medical Research

• Koji Takehiko

  Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences

抄録

B-cell lymphoma 9 (Bcl9) is the core component of Wnt/β-catenin signaling and overexpressed in nuclei of various tumors, including hepatocellular carcinoma (HCC). However, the extent of Bcl9 expression relative to HCC differentiation stage and its functional aspects are poorly understood. In this study, we examined the expression pattern of Bcl9 immunohistochemically, using two anti-Bcl9 antibodies; one was a conventional polyclonal-antibody (anti-Bcl9^ABC) against amino acid no.800–900 of human-Bcl9, while the other (anti-Bcl9^BIO) was against amino acid no.50–200, covering Pygopus-binding sites of Bcl9. Immunohistochemistry using anti-Bcl9^BIO demonstrated distinctive staining in the cytoplasm, while the anti-Bcl9^ABC signal was detected in both cytoplasm and nuclei of HCC cells, reflecting different states of Bcl9 function because Pygopus-binding to Bcl9 is essential to exert its function together with β-catenin in nucleus. Quantitative analysis revealed a significantly higher immunohistochemical-score by anti-Bcl9^BIO in normal liver comparing various differentiation grades of HCC (P < 0.004), whereas no significant difference was noted with anti-Bcl9^ABC. Interestingly, immunohistochemical-score of anti-Bcl9^BIO in patients aged < 40 years was significantly lower than that of ≥ 40 years group (P < 0.01). The results indicated that anti-Bcl9^BIO detected cytoplasmic Bcl9, which does not bind to Pygopus suggesting it could be a useful indicator for development of HCC in young Myanmar patients.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 52 (1), 9-17, 2019-02-28

  日本組織細胞化学会

キーワード

• Bcl9
• Pygopus
• β-catenin
• juvenile
• hepatocellular carcinoma

詳細情報

• CRID: 1390564238078615424
• NII論文ID: 130007605029
• DOI: 10.1267/ahc.18045
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/52/1/52_18045/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2020047137
• 本文言語コード: ja
• データソース種別:
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  • Crossref
  • CiNii Articles
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