Measuring Active Oxygen Species Across a Nonwoven Fabric Using a Pullulan-mixed Methylene Blue Thin Film and Electron Spin Resonance

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Abstract

<p>We successfully developed an active oxygen species (AOS) sensor that reacts only with hydroxyl radicals and is based on pullulan, a water-soluble polymer, and methylene blue. Sterilization with AOS is necessary to use a nonwoven fabric that shields UV light. However, it is difficult to know whether AOS uniformly sterilize in the nonwoven fabric. In this study, an aluminum test box was designed, whose top was sealed with a sterile bag of nonwoven fabric. Hydroxyl-radical-sensing films and spin-trapping agents were placed inside the box, and AOS diffusion and OH* presence was investigated. Experimental results suggest that OH* was not directly generated inside the test box by UV light, in spite of the presence of the nonwoven fabric seal. The OH* present inside the test box sealed with nonwoven fabric is mostly generated either outside the test box by way of UV light irradiation or inside the box by way of ozone decomposition brought about by the reaction of this species with water.</p>

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