テレフタル酸とフェントン反応を利用した河川水中過酸化水素の定量における分析条件の最適化  [in Japanese] Optimization for the Determination of Hydrogen Peroxide in River Water Based on the Fenton Reaction with Terephthalate  [in Japanese]

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Abstract

<p>本研究ではテレフタル酸とフェントン反応を利用した河川水中の過酸化水素の定量法について,反応時間を含む分析時間の短縮と亜硝酸イオンによる感度低下の軽減をめどに,分析条件の最適化を行った.本法では過酸化水素と鉄(II) の反応で生成するヒドロキシルラジカルをテレフタル酸と反応させ,ここから生成する2-ヒドロキシテレフタル酸を蛍光検出高速液体クロマトグラフィーで定量した.反応条件を検討したところ,鉄(II) の濃度を高くすると信号強度は低くなるが,反応時間を短縮できることがわかった.また,反応溶液中に硫酸ナトリウムを添加することで,亜硝酸イオンによる感度低下が軽減できることがわかった.最適化された条件下での検出限界は3-5 nmol L<sup>−1</sup>で,300 nmol L<sup>−1</sup>の過酸化水素標準溶液の繰り返し精度は0.85% であった.広島県東広島市の黒瀬川において連続観測を行ったところ,過酸化水素の濃度は明瞭な日周変化を示し,14 : 00に最大濃度454 nmol L<sup>−1</sup>に達し,日の出前に最小値を示した.</p>

<p>A non-enzymatic fluorescence method for the determination of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) in river water samples was optimized to improve the reaction time and interference by coexisting nitrite. The method was based on the hydroxylation reaction of terephthalate (TP) by a hydroxyl radical formed from a reaction between H<sub>2</sub>O<sub>2</sub> and Fe(II), resulting in the formation of a strongly fluorescent 2-hydroxyterephthalate (HTP). The concentration of Fe(II) strongly affected the reaction time and the signal intensity; the reaction time was shortened by increasing of Fe(II), while the signal intensities decreased with increase Fe(II). The addition of sodium sulfate in the reaction mixture reduced the interference of coexisting nitrite. Under the optimized conditions, a 3-5 nmol L<sup>−1</sup> detection limit and a 0.85 % precision at 300 nmol L<sup>−1</sup> H<sub>2</sub>O<sub>2</sub> were obtained. The diurnal change of hydrogen peroxide was investigated at the Kurose River in Higashi-Hiroshima City, Hiroshima Prefecture. The concentrations of H<sub>2</sub>O<sub>2</sub> were increased in the daytime. The maximum concentration of H<sub>2</sub>O<sub>2</sub> was observed at 14 : 00, while the minimum was observed before sunrise.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 68(2), 125-131, 2019

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130007607786
  • NII NACSIS-CAT ID (NCID)
    AN00222633
  • Text Lang
    JPN
  • ISSN
    0525-1931
  • NDL Article ID
    029529498
  • NDL Call No.
    Z17-9
  • Data Source
    NDL  J-STAGE 
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