Multi-Dimensional HPLC Analysis of Metabolic Related Chiral Amino Acids -Method Development and Biological/Clinical Applications-

• KOGA Reiko

  Faculty of Pharmaceutical Sciences, Fukuoka University

• YOSHIDA Hideyuki

  Faculty of Pharmaceutical Sciences, Fukuoka University

• NOHTA Hitoshi

  Faculty of Pharmaceutical Sciences, Fukuoka University

• HAMASE Kenji

  Graduate School of Pharmaceutical Sciences, Kyushu University

Abstract

The existence of D-amino acids in mammals has been increasingly reported, and clarified that D and L enantiomers have different biological functions, distributions and metabolic pathways. Some D-amino acids were also reported to have a correlation with diseases, and the screening of biomarkers based on quantitative enantioselective metabolomics is highly expected. However, the amounts of most D-amino acids, especially the metabolic-related chiral amino acids, are usually at trace levels in biological samples. Therefore, highly selective and sensitive analytical methods are essential for their accurate determination. In this focusing review, multi-dimensional high-performance liquid chromatographic (HPLC) systems for the precise determination of metabolic-related chiral amino acids are introduced with their application to biological samples. For the highly-sensitive determination, target amino acids were precolumn-derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). The multi-dimensional HPLC systems were composed of a reversed-phase column in the 1^st dimension and an enantioselective column in the 2^nd dimension. By using these systems, various proteinogenic amino acids were clearly observed in the tissues and physiological fluids of rodents. The existence of metabolic-related chiral amino acids in various biological samples were also elucidated.

Journal

• CHROMATOGRAPHY

  CHROMATOGRAPHY 40 (1), 1-8, 2019-02-20

  The Society for Chromatographic Sciences

Keywords

• D-Amino acids
• Multi-dimensional HPLC
• Enantioselective determination
• Metabolic-related amino acids

Details

• CRID: 1390282763104295296
• NII Article ID: 130007611259
• NII Book ID: AA1137755X
• DOI: 10.15583/jpchrom.2019.002
• ISSN: 13483315
• ISSN: 13428284
• NDL BIB ID: 029552436
• Web Site: http://id.ndl.go.jp/bib/029552436
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029552436
• Web Site: https://www.jstage.jst.go.jp/article/jpchrom/40/1/40_2019.002/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed