Species Identification of β-Hemolytic Streptococci from Diseased Companion Animals and Their Antimicrobial Resistance Data in Japan (2017)

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Author(s)

    • Fukushima Yasuto Fukushima Yasuto
    • Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Kitasato Institute for Life Sciences, Kitasato University
    • Tsuyuki Yuzo Tsuyuki Yuzo
    • Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Kitasato Institute for Life Sciences, Kitasato University|Division of Clinical Laboratory, Sanritsu Zelkova Veterinary Laboratory
    • Goto Mieko Goto Mieko
    • Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Kitasato Institute for Life Sciences, Kitasato University
    • Yoshida Haruno Yoshida Haruno
    • Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Kitasato Institute for Life Sciences, Kitasato University

Abstract

<p>This study aimed to identify the species and assess the antimicrobial resistance (AMR) of β-hemolytic streptococci isolated from companion animals in Japan. Strains were isolated from clinical specimens of 131 companion animals that exhibited symptoms in April–May 2017. We identified strains by 16S rRNA sequencing and assessed their antimicrobial susceptibility using the broth microdilution method. AMR genes <i>erm</i>(A)-<i>erm</i>(B)-<i>mef</i>(A) and <i>tet</i>(M)-<i>tet</i>(O)-<i>tet</i>(K)-<i>tet</i>(L)-<i>tet</i>(S) in all isolates were amplified by PCR. 16S rRNA sequencing identified β-hemolytic streptococcal species as <i>Streptococcus canis</i> (<i>n</i> = 117, 89.3%), <i>S. agalactiae</i> (<i>n</i> = 7), <i>S. dysgalactiae</i> subsp. <i>equisimilis</i> (<i>n</i> = 5), <i>S. dysgalactiae</i> subsp. <i>dysgalactiae</i> (<i>n</i> = 1), and <i>S. equi</i> subsp. <i>zooepidemicus</i> (<i>n</i> = 1). Overall AMR rates were 39.7% for minocycline, 19.8% for erythromycin, and 17.6% for clindamycin, with a minimum inhibitory concentration (MIC<sub>90</sub>) of > 4, > 2, and > 1 μg/mL, respectively. AMR genotyping showed the presence of single or mixed types: <i>erm</i>(B)-<i>mef</i>(A) and <i>tet</i>(M)-<i>tet</i>(O)-<i>tet</i>(L)-<i>tet</i>(S). There was a significant relationship between tetracycline-resistance genotypes and open pus/skin-derived specimens. These observations identify some unique features of β-hemolytic streptococcal isolates from companion animals in Japan, such as the dominant isolation of <i>S. canis</i> and resistance to tetracycline, macrolide, and lincosamide antibiotics, in terms of species identification and AMR properties.</p>

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 72(2), 94-98, 2019

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

Codes

  • NII Article ID (NAID)
    130007620386
  • NII NACSIS-CAT ID (NCID)
    AA1132885X
  • Text Lang
    ENG
  • ISSN
    1344-6304
  • NDL Article ID
    029618906
  • NDL Call No.
    Z53-C450
  • Data Source
    NDL  J-STAGE 
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