Potent in vitro Anti-mouth Cancer (KB) and Immunostimulating Activities of the Job's Tears (Coix lachryma-jobi Linn.) Seed Semi-purified Extract Cocktails Containing Linoleic Acid

• Manosroi Jiradej

  Manose Health and Beauty Research Center Faculty of Engineering and Technology, North-Chiang Mai University

• Chankhampan Charinya

  Manose Health and Beauty Research Center Faculty of Engineering and Technology, North-Chiang Mai University

• Kitdamrongtham Worapong

  Manose Health and Beauty Research Center Faculty of Engineering and Technology, North-Chiang Mai University

• Manosroi Worapaka

  Faculty of Medicine, Chiang Mai University

• Manosroi Aranya

  Manose Health and Beauty Research Center Faculty of Engineering and Technology, North-Chiang Mai University Faculty of Pharmacy / Research Administration Center, Chiang Mai University

書誌事項

タイトル別名

• Potent <i>in vitro</i> Anti-mouth Cancer (KB) and Immunostimulating Activities of the Job’s Tears (<i>Coix lachryma-jobi</i> Linn.) Seed Semi-purified Extract Cocktails Containing Linoleic Acid

抄録

The crude methanolic and hexane extracts of non-cooked, steamed and roasted from three Job's Tears cultivars were prepared and further semi-purified by liquid-liquid extraction techniques and silica gel column. The six single semi-purified extracts (F1-F6) were combined as nine cocktails (CT1, CT6, CT8, CT13, CT14, CT21, CT24, CT25 and CT31) according to the IC₅₀ values from the preliminary study and investigated for anti-proliferative and apoptotic induction on mouth cancer cell line (KB) and immunostimulating as well as antioxidative activities. The highest anti-proliferative activity was observed in CT13 showing the IC₅₀ value of 0.53±0.45 µg/mL which was higher than 5-fluorouracil and doxorubicin of 20.34 and 1.60 times, respectively. CT1 which was the combination of F1-F6 and CT13 which was the combination of F4-F6 exhibited significant strong synergistic activity with the combination index value (CI) of 0.28. CT1 at 200 µg/mL showed the highest percentages of apoptotic cells (40.65±10.97%) with no necrotic cells, but lower than cisplatin (100 µg/mL) of 2.18 times. CT14 gave the highest immunostimulating activity with the phagocytosis percentage of 13.0±1.7%, but lower than lipopolysaccharide of 1.08 times. CT31 gave the highest free radical scavenging and metal chelating activities with the SC₅₀ and MC₅₀ values of 0.73±0.07 and 1.99±0.24 µg/mL, but lower than ascorbic acid and EDTA of 18.25 and 4.33 times, respectively. The linoleic acid contents related to anti-cancer activity were also examined by HPLC. This study has demonstrated that CT1 composing of F1-F6 at the percentage ratio of 0.71:2.06:81.38: 8.47:4.92:2.46 was the potential cocktails of the semi-purified extracts from the Job’s Tears which can be further developed as a novel active compound for oral cancer treatment.

収録刊行物

• Journal of Oleo Science

  Journal of Oleo Science 68 (4), 351-359, 2019

  公益社団法人 日本油化学会

キーワード

• semi-purified extracts
• Job’s Tears
• anti-cancer
• immunostimulating
• linoleic acid

詳細情報

• CRID: 1390001288143720832
• NII論文ID: 130007621724
• NII書誌ID: AA11503337
• DOI: 10.5650/jos.ess18255
• ISSN: 13473352
• ISSN: 13458957
• NDL書誌ID: 029604438
• PubMed: 30930371
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029604438
• Web Site: https://www.jstage.jst.go.jp/article/jos/68/4/68_ess18255/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2019319528
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可