The Journal of Nihon University School of Dentistry <i>In vivo</i> antimicrobial activity of silver diammine fluoride on carious lesions in dentin

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Author(s)

    • Karched Maribasappa
    • Oral Microbiology Research Laboratory, Department of Bioclinical Sciences, Faculty of Dentistry, Kuwait University
    • Ali Dena
    • Department of General Dental Practice, Faculty of Dentistry, Kuwait University
    • Ngo Hien
    • Department of General Dental Practice, Faculty of Dentistry, Kuwait University

Abstract

<p>Minimally invasive treatment protocols may leave a residual layer of carious dentin, which requires treatment for the inhibition of bacterial growth beneath restorations. We aimed to examine the <i>in vivo</i> effect of silver diammine fluoride (SDF) and SDF + potassium iodide (KI) application on bacteria present in deep carious lesions. We studied the <i>in vivo</i> efficacy in five patients, each of which had five carious lesions. Dentin samples taken before and after treatment were subjected to microbial analyses. Following treatment with SDF, the median colony-forming unit (CFU) counts per mg of dentin reduced from 9 × 10<sup>5</sup> to 1.6 × 10<sup>2</sup> (<i>P</i> < 0.05), and following that with SDF + KI, the counts decreased from 2.9 × 10<sup>5</sup> to 9.2 × 10 (<i>P</i> < 0.05). The use of chlorhexidine gluconate (CHX) reduced CFU counts from 1.1 × 10<sup>5</sup> to 4.8 × 10<sup>2</sup> (<i>P</i> < 0.05). In four of the five patients, no CFUs were found on mitis salivarius-bacitracin agar with respect to SDF or SDF + KI application. For CHX, the median CFU count before treatment was 1.6 × 10<sup>3</sup> and that after treatment was 1.1 × 10<sup>2</sup>. SDF completely inhibited mutans streptococci growth in four of the five patients, while the growth of anaerobes was not completely inhibited.</p>

Journal

  • Journal of Oral Science

    Journal of Oral Science 61(1), 19-24, 2019

    Nihon University School of Dentistry

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