Impact of Introduction of Arbuscular Mycorrhizal Fungi on the Root Microbial Community in Agricultural Fields

• Akyol Turgut Yigit

  Graduate School of Life Sciences, Tohoku University

• Niwa Rieko

  Central Region Agricultural Research Center, National Agriculture and Food Research Organization (NARO)

• Hirakawa Hideki

  Kazusa DNA Research Institute

• Maruyama Hayato

  Graduate School of Agriculture, Hokkaido University

• Sato Takumi

  Faculty of Agriculture, Yamagata University

• Suzuki Takae

  Field Science Center, Graduate School of Agriculture, Tohoku University

• Fukunaga Ayako

  Western Region Agricultural Research Center, NARO

• Sato Takashi

  Faculty of Bioresource Sciences, Akita Prefectural University

• Yoshida Shigenobu

  Central Region Agricultural Research Center, National Agriculture and Food Research Organization (NARO)

• Tawaraya Keitaro

  Faculty of Agriculture, Yamagata University

• Saito Masanori

  Field Science Center, Graduate School of Agriculture, Tohoku University Department of Innovation Research, Japan Science and Technology Agency

• Ezawa Tatsuhiro

  Graduate School of Agriculture, Hokkaido University

• Sato Shusei

  Graduate School of Life Sciences, Tohoku University

抄録

Arbuscular mycorrhizal (AM) fungi are important members of the root microbiome and may be used as biofertilizers for sustainable agriculture. To elucidate the impact of AM fungal inoculation on indigenous root microbial communities, we used high-throughput sequencing and an analytical pipeline providing fixed operational taxonomic units (OTUs) as an output to investigate the bacterial and fungal communities of roots treated with a commercial AM fungal inoculum in six agricultural fields. AM fungal inoculation significantly influenced the root microbial community structure in all fields. Inoculation changed the abundance of indigenous AM fungi and other fungal members in a field-dependent manner. Inoculation consistently enriched several bacterial OTUs by changing the abundance of indigenous bacteria and introducing new bacteria. Some inoculum-associated bacteria closely interacted with the introduced AM fungi, some of which belonged to the genera Burkholderia, Cellulomonas, Microbacterium, Sphingomonas, and Streptomyces and may be candidate mycorrhizospheric bacteria that contribute to the establishment and/or function of the introduced AM fungi. Inoculated AM fungi also co-occurred with several indigenous bacteria with putative beneficial traits, suggesting that inoculated AM fungi may recruit specific taxa to confer better plant performance. The bacterial families Methylobacteriaceae, Acetobacteraceae, Armatimonadaceae, and Alicyclobacillaceae were consistently reduced by the inoculation, possibly due to changes in the host plant status caused by the inoculum. To the best of our knowledge, this is the first large-scale study to investigate interactions between AM fungal inoculation and indigenous root microbial communities in agricultural fields.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 34 (1), 23-32, 2019

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• root microbiome
• high-throughput community analysis
• network analysis
• microbiome manipulation
• sustainable agriculture

詳細情報

• CRID: 1390564238094773632
• NII論文ID: 130007627738
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me18109
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 029596392
• PubMed: 30584188
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029596392
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/34/1/34_ME18109/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可