The Journal of Nihon University School of Dentistry The effect of mesenchymal stem cells on osteoclast precursor cell differentiation

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Author(s)

    • Abe Takaharu
    • Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital
    • Sumi Keisuke
    • Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital
    • Kunimatsu Ryo
    • Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital
    • Oki Nanae
    • Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital
    • Tsuka Yuji
    • Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital
    • Nakajima Kengo
    • Department of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences
    • Ando Kazuyo
    • Department of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences
    • Tanimoto Kotaro
    • Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital

Abstract

<p>Transplantation of mesenchymal stem cells (MSCs) has been extensively studied in the field of regenerative medicine. Bone regeneration is achieved via the interaction of osteoblasts and osteoclasts. However, the influence of MSCs on osteoclasts is unknown. The purpose of this study was to investigate the effect of MSCs on the expression of genes for osteoclast differentiation factors using qPCR after indirect co-culture of MSCs and RAW264 cells. The numbers of osteoclasts after addition of soluble receptor activator of nuclear factor kappa B (NF-κB) ligand (sRANKL) were also compared. Expression of osteoprotegerin (OPG) by MSCs was significantly elevated in co-culture over time. The differentiation of RAW264 cells into mature osteoclasts following addition of sRANKL was significantly inhibited by co-culture with MSCs. Expression of RANK, colony stimulating factor 1 receptor, NF-κB, and nuclear factor of activated T-cell cytoplasmic 1 in RAW264 cells was significantly inhibited by co-culture with MSCs. Expression of OPG protein was higher in co-culture with RAW264 cells than in MSCs alone, and the expression level was clearly higher than that of RANKL. MSCs appeared to inhibit osteoclast differentiation via expression of OPG.</p>

Journal

  • Journal of Oral Science

    Journal of Oral Science 61(1), 30-35, 2019

    Nihon University School of Dentistry

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