Effect of Concentration and Time on the Antimicrobial Activity of Human Saliva against <i>Candida albicans </i>

DOI Web Site 参考文献7件
  • Ono Masanori
    Nihon University Graduate school of Dentistry at Matsudo, Removable Prosthodontics, Matsudo, Chiba 271-8587, Japan
  • Watanabe Takehiro
    Department of Removable Prosthodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan
  • Kimoto Suguru
    Department of Removable Prosthodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan
  • Kobayashi Ryoki
    Department of Community Oral Health, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan
  • Kono Tetsuro
    Department of Histology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan
  • Kurita-Ochiai Tomoko
    Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan
  • Kawai Yasuhiko
    Department of Removable Prosthodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

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<p>Mechanical cleaning is considered most effective in preventing oral mucosal diseases such as denture stomatitis and aspiration pneumonia; however, host saliva also have effective antimicrobial substances. Beta-defensin and histatin reportedly exert antimicrobial effects against Candida albicans; however, their optimal concentration in human saliva and optimum duration of activity are unclear. Therefore, this study aimed to investigate the effects of concentration and time on the antimicrobial activity of human saliva against C. albicans. Saliva was collected from 20 volunteers(10 men, 10 women; 25.7±1.95 years) twice daily for 1 week, and each antimicrobial substances was quantified. The viable count,ATP activity, and changes in the drug susceptibility test for C. albicans were assessed with respect to different concentrations and durations to analyse antimicrobial activity. Viable count tended to increase with an increase in beta-defensin 3 levels, but tended to decrease with an increase in histatin 5 levels. Yeast density and ATP activity level were significantly positively correlated with each antimicrobial substance(beta-defensin 3; 0.991, p＜.000,histatin 5; 0.975, p＜.000). Beta-defensin 3 and histatin 5 did not yield zones of inhibition with time; however, antifungal drugs reduced the viable count and ATP activity level regardless of time. The present results indicate that histatin 5 reduces the population of C. albicans and ATP activity levels and may be a potential marker for risk assessment of oral mucosal diseases and aspiration pneumonia.</p>

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  • ＩＪＯＭＳ

    ＩＪＯＭＳ 17 (3-4), 114-119, 2019-03-20

    日本大学松戸歯学部 口腔科学研究所

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