Ex Vivo Method to Simultaneously Evaluate Glucose Utilization, Uptake, and Production in Rat Liver

• TSUMURA Yoshinori

  Pharmaceutical Development Coordination Department, Teijin Pharma Limited

• TSUSHIMA Yu

  Pharmacology Research Department, Teijin Institute for Bio-medical Research, Teijin Pharma Limited

• TAMURA Azusa

  Pharmacology Research Department, Teijin Institute for Bio-medical Research, Teijin Pharma Limited

• HASEBE Makiko

  Pharmacology Research Department, Teijin Institute for Bio-medical Research, Teijin Pharma Limited

• KOBAYASHI Tsunefumi

  Pharmacology Research Department, Teijin Institute for Bio-medical Research, Teijin Pharma Limited

書誌事項

タイトル別名

• <i>Ex Vivo</i> Method to Simultaneously Evaluate Glucose Utilization, Uptake, and Production in Rat Liver

抄録

A novel ex vivo method to simultaneously evaluate hepatic glucose utilization, uptake, and production was developed in rats. The right lateral lobe of the liver was perfused with Krebs–Henseleit bicarbonate buffer containing 5 mmol/L uniformly labeled ¹³C-glucose ([U-¹³C]-glucose). The whole glucose concentration in the perfusate was measured by colorimetric assay, and the concentrations of [U-¹²C]-glucose (natural isotope) or [U-¹³C]-glucose were estimated on the basis of the abundance ratio of [U-¹²C]-glucose or [U-¹³C]-glucose, which were measured by GC-MS. The difference in whole glucose and [U-¹³C]-glucose concentrations between the baseline and effluent perfusate represents hepatic glucose utilization and glucose uptake, respectively. The [U-¹²C]-glucose concentration in the effluent perfusate corresponds to hepatic glucose production. With this method, we clarified the precise mechanism that underlies the hepatic impairment of diabetic animals and pharmacological effects of anti-diabetic agents. Thus, this method is useful for the pathophysiological and pharmacological research of type 2 diabetes.

収録刊行物

• Analytical Sciences

  Analytical Sciences 35 (4), 455-460, 2019-04-10

  社団法人 日本分析化学会

キーワード

• Hepatic glucose metabolism
• gas chromatography
• mass spectrometry
• diabetes mellitus
• partial liver perfusion

詳細情報

• CRID: 1390001288131083392
• NII論文ID: 130007629983
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.18p427
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 029626058
• PubMed: 30643097
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029626058
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.18P427.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.18P427/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/35/4/35_18P427/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可