Lycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species

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  • YANG Lei
    College of Basic Medical Science, Jiujiang University, Jiangxi 332000, China Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiangxi 332000, China
  • GAO Zhen
    Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Shaanxi 712100, China
  • LEI Lanjie
    Affiliated Hospital of Jiujiang University, Jiujiang University, Jiangxi 332000, China
  • LV Qizhuang
    College of Biology & Pharmacy, Yulin Normal University, Guangxi 53700, China
  • ZHAO Qihan
    Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiangxi 332000, China
  • LI Lixin
    College of Basic Medical Science, Jiujiang University, Jiangxi 332000, China
  • CAO Xiaoming
    College of Basic Medical Science, Jiujiang University, Jiangxi 332000, China
  • FU Wenxue
    College of Basic Medical Science, Jiujiang University, Jiangxi 332000, China Key Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiangxi 332000, China

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  • <i>Lycium barbarum</i> polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species

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<p>Lycium barbarum polysaccharide (LBP) exhibits multiple pharmacological and biological effects, including displaying antioxidant and cytoprotective properties. The current study investigated the effects of LBP-supplemented culture medium on mitochondrial distribution, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) production, mitochondrial deoxyribonucleic acid (mtDNA) copy number, reactive oxygen species (ROS) accumulation, and development of previously-cryopreserved murine two-cell embryos. Results indicate that LBP enhances development of such embryos, and that potential mechanisms include: (1) mitochondrial function enhancement via altering mitochondrial distribution and increasing MMP, ATP production, mtDNA copy number, and expression of genes involved in mitochondrial biogenesis and energy metabolism (NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and phosphorylated adenosine monophosphate-activated protein kinase (pAMPK)); (2) down-regulation of ROS generation and enhanced expression of the antioxidant genes glutathione peroxidase 4 (GPX4) and superoxide dismutase 1 (SOD1), thereby increasing embryo oxidative stress tolerance; and (3) increased expression of B-cell lymphoma-2 (BCL2), a critical gene for cell survival and embryo development. These results demonstrate that LBP improves development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of ROS.</p>

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