High rate misidentification of biochemically determined <i>Streptococcus</i> isolates from swine clinical specimens
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- MEEKHANON Nattakan
- Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand
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- KAEWMONGKOL Sarawan
- Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand
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- JIRAWATTANAPONG Pichai
- Department of Farm Resources and Production Medicines, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand
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- KAMINSONSAKUL Tanyanant
- Kamphaengsaen Veterinary Diagnostic Unit, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand
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- KONGSOI Siriporn
- Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand
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- CHUMSING Suksan
- Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand
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- OKURA Masatoshi
- Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan
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- UENO Yuichi
- Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan
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- SEKIZAKI Tsutomu
- Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- TAKAMATSU Daisuke
- Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu 501-1193, Japan
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Abstract
<p>In this study, 22 bacterial isolates from swine necropsy specimens, which were biochemically identified as Streptococcus suis and other Streptococcus species, were re-examined using species-specific PCR for authentic S. suis and 16S rRNA gene sequencing for the verification of the former judge. Identification of S. suis on the basis of biochemical characteristics showed high false-positive (70.6%) and false-negative (60%) rates. The authentic S. suis showed various capsular polysaccharide synthesis gene types, including type 2 that often isolated from human cases. Five of 22 isolates did not even belong to the genus Streptococcus. These results suggested that the misidentification of the causative pathogen in routine veterinary diagnosis could be a substantial obstacle for the control of emerging infectious diseases.</p>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 81 (4), 567-572, 2019
JAPANESE SOCIETY OF VETERINARY SCIENCE