Electron Microscopic Study on the Formation of Hardened Molds of Calcium Phosphate Crystals Containing Gelatin

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Author(s)

    • Tai Yasuharu
    • Department of Endodontology, Tsurumi University School of Dental Medicine
    • Asada Yuka
    • Department of Operative Dentistry, Tsurumi University School of Dental Medicine
    • Chiba Toshie
    • Department of Oral Anatomy, Tsurumi University School of Dental Medicine
    • Miake Yasuo
    • Department of Histology and Developmental Biology, Tokyo Dental College
    • Shimoda Shinji
    • Department of Oral Anatomy, Tsurumi University School of Dental Medicine
    • Hosoya Noriyasu
    • Department of Endodontology, Tsurumi University School of Dental Medicine

Abstract

<p>Fundamental studies on biocompatible materials were conducted using the mechanism of dentin formation as a model. Hydroxyapatite and fluorinated apatite were synthesized in a gelatin solution. Synthesized apatite was pressed under vacuum, and the mechanical strength was investigated. A hardened mold was prepared by adding amorphous calcium phosphate (ACP). Then, changes in the crystals of the hardened mold dipped in distilled water or 1 ppm fluoride solution were observed with a transmission electron microscope. The results showed that the crystal growth of apatite synthesized in 1% gelatin was suppressed. Moreover, small and homogenous granular apatite was formed in the precipitate synthesized with fluorine. Gelatin contributed to the reduction of brittleness of the hardened mold, and thus a high cutting resistance value was obtained. Furthermore, acceleration of crystal growth and aggregation of fine crystals were observed in the hardened mold when ACP was added to the solution.</p>

Journal

  • Journal of Hard Tissue Biology

    Journal of Hard Tissue Biology 28(2), 165-174, 2019

    THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY

Codes

  • NII Article ID (NAID)
    130007636419
  • Text Lang
    ENG
  • ISSN
    1341-7649
  • Data Source
    J-STAGE 
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