Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in <i>Bacillus subtilis</i> cells lacking lipoteichoic acid
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- Seki Takahiro
- Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University
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- Furumi Takuya
- Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University
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- Hashimoto Michihiro
- Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University
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- Hara Hiroshi
- Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University
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- Matsuoka Satoshi
- Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University
Bibliographic Information
- Other Title
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- Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid
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Abstract
<p>In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA). LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane. Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA. Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone. Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively). These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.</p>
Journal
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- Genes & Genetic Systems
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Genes & Genetic Systems 94 (2), 71-80, 2019-04-01
The Genetics Society of Japan
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Details 詳細情報について
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- CRID
- 1390282763110864768
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- NII Article ID
- 130007638723
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- NII Book ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL BIB ID
- 029670578
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- PubMed
- 30971625
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed