The Journal of Toxicological Sciences Transcriptional profiling of cytochrome P450 genes in the liver of adult zebrafish, <i>Danio rerio</i>

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Author(s)

    • Kubota Akira
    • Laboratory of Toxicology, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
    • Teraoka Hiroki
    • Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Rakuno Gakuen University
    • Kawai Yusuke K.
    • Laboratory of Toxicology, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
    • Yamashita Natsumi
    • Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Rakuno Gakuen University
    • Lee Jae Seung
    • Laboratory of Toxicology, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
    • Kondoh Daisuke
    • Laboratory of Veterinary Anatomy, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
    • Zhang Shuangyi
    • Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Rakuno Gakuen University
    • Nishi Yasunobu
    • Department of Large Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University
    • Suzuki Kazuyuki
    • Department of Large Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University
    • Kitazawa Takio
    • Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Rakuno Gakuen University

Abstract

<p>Increasing use of zebrafish in biomedical, toxicological and developmental studies requires explicit knowledge of cytochrome P450 (CYP), given the central role of CYP in oxidative biotransformation of xenobiotics and many regulatory molecules. A full complement of <i>CYP</i> genes in zebrafish and their transcript expression during early development have already been examined. Here we established a comprehensive picture of <i>CYP</i> gene expression in the adult zebrafish liver using a RNA-seq technique. Transcriptional profiling of a full complement of <i>CYP</i> genes revealed that <i>CYP2AD2</i>, <i>CYP3A65</i>, <i>CYP1A</i>, <i>CYP2P9</i> and <i>CYP2Y3</i> are major <i>CYP</i> genes expressed in the adult zebrafish liver in both sexes. Quantitative real-time RT-PCR analysis for selected <i>CYP</i> genes further supported our RNA-seq data. There were significant sex differences in the transcript levels for <i>CYP1A</i>, <i>CYP1B1</i>, <i>CYP1D1</i> and <i>CYP2N13</i>, with males having higher expression levels than those in females in all cases. A similar feature of gender-specific expression was observed for <i>CYP2AD2</i> and <i>CYP2P9</i>, suggesting sex-specific regulation of constitutive expression of some <i>CYP</i> genes in the adult zebrafish liver. The present study revealed several "orphan" <i>CYP</i> genes as dominant isozymes at transcript levels in the adult zebrafish liver, implying crucial roles of these <i>CYP</i> genes in liver physiology and drug metabolism. The current results establish a foundation for studies with zebrafish in drug discovery and toxicology.</p>

Journal

  • The Journal of Toxicological Sciences

    The Journal of Toxicological Sciences 44(5), 347-356, 2019

    The Japanese Society of Toxicology

Codes

  • NII Article ID (NAID)
    130007642915
  • Text Lang
    ENG
  • ISSN
    0388-1350
  • Data Source
    J-STAGE 
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