Using Electropolymerization-based Doping for the Electro-addressable Functionalization of a Multi-electrode Array Probe for Nucleic Acid Detection

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Author(s)

    • SEN Mustafa SEN Mustafa
    • Biomedical Engineering Department, Izmir Katip Celebi University|Biomedical Technologies Graduate Program, Izmir Katip Celebi University

Abstract

<p>Even though large number of individually addressable electrodes can be effectively assembled in a small area, electrochemical detection methods have a relatively limited ability to detect multiple analytes compared to microdialysis probes and other analytic techniques. Here, we report a facile method for the electro-addressable functionalization of a probe comprising of closely spaced three individually addressable carbon fiber electrodes (CFEs) for the detection of nucleic acids. First, a multi electrode array probe comprising three adjacent CFEs was fabricated through pulling a three-barrel glass capillary with a single carbon fiber in each barrel. Second, electropolymerization based doping was used for the electro-addressable functionalization of the multi-electrode array probe. To demonstrate that the current strategy works, anti-miR-34a was electrografted on only one of three electrodes by the electropolymerization of pyrrole on a specific electrode. A second electrode was coated only with polypyrrole (PPy) and the third was left unmodified. The results demonstrate that the present strategy has great potential for constructing multiplex nucleic acid micro/nano biosensors for local and <i>in situ</i> detection of multiple nucleic acid molecules, such as miRNAs at a time.</p>

Journal

  • Analytical Sciences

    Analytical Sciences 35(5), 565-569, 2019

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130007644153
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • ISSN
    0910-6340
  • NDL Article ID
    029674374
  • NDL Call No.
    Z54-F482
  • Data Source
    NDL  J-STAGE 
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