Attenuated Airway Eosinophilic Inflammations in IL-38 Knockout Mouse Model

• MATSUOKA MASANOBU

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

• KAWAYAMA TOMOTAKA

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

• TOMINAGA MASAKI

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

• KAIEDA SHINJIRO

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

• TOKUNAGA YOSHIHISA

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

• KAKU YOICHIRO

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

• IMAOKA HARUKI

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

• KINOSHITA TAKASHI

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

• OKAMOTO MASAKI

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

• AKIBA JUN

  Department of Diagnostic Pathology, Kurume University Hospital

• HOSHINO TOMOAKI

  Division of Respirology, Neurology and Rheumatology, Department of Internal Medicine, Kurume University School of Medicine

Abstract

Summary: Background: The role of IL-38, a new member of the IL-1 family, in airway eosinophilic inflammatory conditions such as asthma is unclear. To investigate the role of IL-38 in airway eosinophilic inflammation, an IL-38-gene deficient (KO) murine asthma model was analyzed. Methods: The numbers of eosinophils and neutrophils, and levels of IL-5, IL-13 and IL-17A protein and mRNA in bronchoalveolar lavage fluid (BALF) and lung tissue were compared between wild-type (WT) and IL-38-KO mice after OVA sensitization and challenge. The effects of additional purified recombinant mouse (rm) IL-38 protein were investigated in the IL-38-KO murine asthma model. Results: The IL-38 and IL-5 mRNA in WT mice was significantly higher after OVA challenge than after saline challenge (p<0.05). The number of airway eosinophils in IL-38-KO mice was significantly lower than in WT mice after OVA challenge (p<0.01). BALF analysis confirmed the lower number of airway eosinophils in IL-38-KO mice and showed that this was significantly associated with lower IL-5 protein levels (r=0.92, p<0.0001). However, the additional rm IL-38 protein did not neutralize airway eosinophilia in IL-38-KO mice. Conclusion: IL-38 may enhance airway eosinophilic inflammation in asthma through IL-5 induction.

Journal

• The Kurume Medical Journal

  The Kurume Medical Journal 65 (2), 37-46, 2018-06-30

  Kurume University School of Medicine

Keywords

• Airway inflammation
• Cytokine
• Asthm
• IL-38
• Eosinophils

Details

• CRID: 1390845713068747264
• NII Article ID: 130007650022
• DOI: 10.2739/kurumemedj.ms652009
• ISSN: 18812090
• ISSN: 00235679
• PubMed: 30853691
• Web Site: https://www.jstage.jst.go.jp/article/kurumemedj/65/2/65_MS652009/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2020029253
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed