The neutral <i>N</i>-linked glycans of the Basidiomycetous yeasts <i>Pseudozyma antarctica</i> and <i>Malassezia furfur</i> (Subphylum Ustilaginomycotina)

• Flores Ronilo Jose D.

  International Center for Biotechnology, Osaka University

• Ohashi Takao

  International Center for Biotechnology, Osaka University

• Sakai Kanae

  Medical Mycology Research Center, Chiba University

• Gonoi Tohru

  Medical Mycology Research Center, Chiba University

• Kawasaki Hiroko

  NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE)

• Fujiyama Kazuhito

  International Center for Biotechnology, Osaka University

抄録

Pseudozyma antarctica and Malassezia furfur are basidiomycetous yeasts under the subphylum Ustilaginomycotina. P. antarctica is a commensal organism found in certain plant species, while M. furfur is associated with several skin diseases of animals including humans. N-linked glycans of P. antarctica and M. furfur were prepared, digested with glycosidases, and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS). Analyses revealed the presence of neutral N-linked glycans ranging in length from Man₃GlcNAc₂-PA to Man₉GlcNAc₂-PA. The two species shared the most abundant neutral N-linked glycan: Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M8A). The second and third most abundant neutral N-linked glycans for P. antarctica were Manα1-2Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M9A) and Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M5A), respectively. In the case of M. furfur, Manα1-2Manα1-6(Manα1-3)Manα1-6(Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc (M7A) was the second most abundant, while both M8A and M9A were tied for the third most abundant. The presence of putative galactose residues in the hypermannosylated neutral N-linked glycans is also discussed. This report is the first to analyze the neutral N-linked glycans of P. antarctica and M. furfur.

収録刊行物

• The Journal of General and Applied Microbiology

  The Journal of General and Applied Microbiology 65 (2), 53-63, 2019

  公益財団法人　応用微生物学・分子細胞生物学研究奨励会

キーワード

• Basidiomycetous yeasts
• <i>Malassezia</i>
• <i>N</i>-linked glycan
• <i>Pseudozyma</i>

詳細情報

• CRID: 1390564238091367680
• NII論文ID: 130007651708
• DOI: 10.2323/jgam.2018.05.003
• ISSN: 13498037
• ISSN: 00221260
• PubMed: 30305477
• Web Site: https://www.jstage.jst.go.jp/article/jgam/65/2/65_2018.05.003/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可