マイクロチャネルデバイスを用いた細胞遊走メカニズムの解明

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  • Investigation of cell migration mechanism by using microchannel device

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<p>Cell migration of mechanically coupled cell layers is essential for a variety of biological and pathological processes such as embryonic development, wound healing, inflammation and tumor metastasis. However, the mechanism of the collective cell migration has not been characterized well. In this study, a polydimethylsiloxane-made microchannel device was fabricated using photolithography and soft lithography technology, and was used to monitor traction forces orientation generated by cells during migration. The device consists of a reservoir for establishing a confluent cell monolayer which connects with microchannel. The collective cell would migrate into channel initiated by the backfilling of medium. The microchannel had a width of 200 μm, equipped with micropillars whose dimension were 6.5μm in height, 3μm in diameter and 7μm in spacing. As the result, migration rate of 10.7 um/h and spread rate of 291.6 μm2/h was measured during cells migration, average traction force for one pillar was measure by 12.1 nN, and maximal traction force orientation rotation by counterclockwise with a rate of 0.3 rad/h.</p>

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