Dietary daidzein induces accumulation of <i>S</i>-equol in enterohepatic circulation to far higher levels than that of daidzein in female rats with and without ovariectomy
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- FUJITANI Mina
- Graduate School of Agriculture, Ehime University
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- MIZUSHIGE Takafumi
- Department of Applied Biological Chemistry, Faculty of Agriculture, Utsunomiya University
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- BHATTARAI Keshab
- Graduate School of Agriculture, Ehime University The United Graduate School of Agricultural Sciences, Ehime University
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- ADHIKARI Sudhashree
- The United Graduate School of Agricultural Sciences, Ehime University
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- ISHIKAWA Junji
- Central Research and Development Laboratory, FANCL Co. Ltd.
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- KISHIDA Taro
- Graduate School of Agriculture, Ehime University The United Graduate School of Agricultural Sciences, Ehime University
Bibliographic Information
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- Dietary daidzein induces accumulation of S-equol in enterohepatic circulation to far higher levels than that of daidzein in female rats with and without ovariectomy
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Abstract
<p>We previously found that daidzein decreased food intake in female rats. To understand the mechanism of anorectic action of dietary daidzein, it is necessary to determine distributions of daidzein and S-equol, a metabolite of intestinal bacterial conversion from daidzein, in the body. In the present study, we measured the concentrations of daidzein and S-equol in serum and bile in sham-operated and ovariectomized female rats fed a diet containing 150 mg/kg daidzein for 7 days. Dietary daidzein increased serum and bile concentrations of S-equol to far higher levels than those of daidzein. S-equol concentration was more than several hundred fold-higher in bile than in serum, regardless of ovariectomy. Moreover, to investigate whether accumulation of S-equol is facilitated by efficient enterohepatic circulation during continuous intake of daidzein and S-equol, female rats were fed diet containing daidzein or S-equol (both 150 mg/kg), or control diet for 1, 2, 3, or 5 days. Dietary daidzein significantly increased serum and bile concentrations of S-equol in a time-dependent manner, but not those of daidzein. These results indicated that substantial proportion of dietary daidzein was converted to S-equol, which underwent efficient enterohepatic circulation and predominantly accumulated there.</p>
Journal
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- Biomedical Research
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Biomedical Research 40 (3), 97-105, 2019-06-01
Biomedical Research Press