植物における一過的タンパク質大量発現系「つくばシステム」について  [in Japanese] Efficient transient protein expression in plants, 'Tsukuba system'  [in Japanese]

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Author(s)

    • 三浦 謙治 Miura Kenji
    • 筑波大学生命環境系/つくば機能植物イノベーション研究センター Faculty of Life and Environmental Sciences/ Tsukuba-Plant Innovation Research Center (T-PIRC), University of Tsukuba

Abstract

<p>To produce recombinant proteins and identify the gene functions, such as subcellular localization, one of the strongest agro-infiltration-based transient protein expression system for plant cells, termed 'Tsukuba system' has been established. By using this system, about 4 mg/g fresh weight of protein expression was detected in <i>Nicotiana benthamiana.</i> Furthermore, the system is also applicable to various plant species, including eggplant, tomato cultivars and wild species, lettuce, hot pepper, soybean, common bean, and orchid. The vector pBYR2HS contains a geminiviral replication system and a double terminator. Thus, the system is an effective and valuable tool for rapid transient expression to obtain a bunch of proteins and to examine gene function.</p>

<p>To produce recombinant proteins and identify the gene functions, such as subcellular localization, one of the strongest agro-infiltration-based transient protein expression system for plant cells, termed 'Tsukuba system' has been established. By using this system, about 4 mg/g fresh weight of protein expression was detected in <i>Nicotiana benthamiana.</i> Furthermore, the system is also applicable to various plant species, including eggplant, tomato cultivars and wild species, lettuce, hot pepper, soybean, common bean, and orchid. The vector pBYR2HS contains a geminiviral replication system and a double terminator. Thus, the system is an effective and valuable tool for rapid transient expression to obtain a bunch of proteins and to examine gene function.</p>

Journal

  • Regulation of Plant Growth & Development

    Regulation of Plant Growth & Development 54(1), 77-81, 2019

    The Japanese Society for Chemical Regulation of Plants

Codes

  • NII Article ID (NAID)
    130007668430
  • NII NACSIS-CAT ID (NCID)
    AA11550064
  • Text Lang
    JPN
  • ISSN
    1346-5406
  • NDL Article ID
    029776063
  • NDL Call No.
    Z18-67
  • Data Source
    NDL  J-STAGE 
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