植物組織培養 Agroinfiltration-based efficient transient protein expression in leguminous plants

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Author(s)

    • Suzaki Takuya
    • Faculty of Life and Environmental Sciences, University of Tsukuba|Tsukuba-Plant Innovation Research Center (T-PIRC), University of Tsukuba
    • Tsuda Mai
    • Faculty of Life and Environmental Sciences, University of Tsukuba|Tsukuba-Plant Innovation Research Center (T-PIRC), University of Tsukuba
    • Ezura Hiroshi
    • Faculty of Life and Environmental Sciences, University of Tsukuba|Tsukuba-Plant Innovation Research Center (T-PIRC), University of Tsukuba
    • Day Brad
    • Department of Plant, Soil and Microbial Sciences, Michigan State University|Michigan State University Plant Resilience Institute
    • Miura Kenji
    • Faculty of Life and Environmental Sciences, University of Tsukuba|Tsukuba-Plant Innovation Research Center (T-PIRC), University of Tsukuba

Abstract

<p>Transient protein expression is an effective tool to rapidly unravel novel gene functions, such as transcriptional activity of promoters and sub-cellular localization of proteins. However, transient expression is not applicable to some species and varieties because of insufficient expression levels. We recently developed one of the strongest agroinfiltration-based transient protein expression systems for plant cells, termed 'Tsukuba system.' About 4 mg/g fresh weight of protein expression in <i>Nicotiana benthamiana</i> was obtained using this system. The vector pBYR2HS, which contains a geminiviral replication system and a double terminator, can be used in various plant species and varieties, including lettuces, eggplants, tomatoes, hot peppers, and orchids. In this study, we assessed the applicability of the Tsukuba system to several species of legumes, including <i>Lotus japonicus</i>, soybean <i>Glycine max</i>, and common bean <i>Phaseolus vulgaris</i>. The GFP protein was transiently expressed in the seedpods of all examined legume species, however, protein expression in leaves was observed only in <i>P. vulgaris</i>. Taken together, our system is an effective tool to examine gene function rapidly in several legume species based on transient protein expression.</p>

Journal

  • Plant Biotechnology

    Plant Biotechnology 36(2), 119-123, 2019

    Japanese Society for Plant Cell and Molecular Biology

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