A simple preparation method for observation of aquatic micro-organisms by scanning electron microscope

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  • Inoue M.
    Department of Electrical & Electronic Engineering, Faculty of Science & Engineering, Setsunan University
  • Suganami M.
    Academic Support Center, Setsunan University

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  • 走査電子顕微鏡を用いた水生微生物観察のための簡易試料前処理法
  • ソウサ デンシ ケンビキョウ オ モチイタ スイセイ ビセイブツ カンサツ ノ タメ ノ カンイ シリョウ マエショリホウ

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Abstract

For observation of aquatic micro-organisms by a scanning electron microscope, sample pretreatments are necessary to avoid the deformation of cell membrane by evaporation of moisture from inside of the cell in vacuum and also the charging-up of sample under irradiation of electron beam. In the present paper, we report on a simple preparation method by ethanol dehydration and the ionic liquid coating method without using any apparatus for preparation such as a refrigerator, a vacuum equipment and etc.

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