Bioluminescence Microplate Assay of Cyanide with <i>Escherichia coli</i> Harboring a Plasmid Responsible for Cyanide-dependent Light Emission in Alginate Microenvironment

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Author(s)

Abstract

<p>We describe the bioluminescence of a genetically engineered <i>Escherichia coli</i> harboring a recombined plasmid with a catalase gene promoter fused <i>lux</i> gene cluster, responsible for the generation of photons closely associated with respiratory inhibition, with the aim of applying it for cyanide sensing. This <i>E. coli</i> construct was favorably utilized for the microplate assay of cyanide by leveraging the microenvironment of the biocompatible alginate. The brightness of the bioluminescence, induced by cyanide stimulation of the respiration causative of the production of hydrogen peroxide, positively correlates with its concentration. Moreover, visualization of cyanide with a consumer digital camera, ranging in concentration from about 0.01 mg CN·L<sup>−1</sup> in the alginate sol to around 100 mg CN·L<sup>−1</sup> in its gel, was attained.</p>

Journal

  • Analytical Sciences

    Analytical Sciences 35(7), 821-825, 2019

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130007676976
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • ISSN
    0910-6340
  • NDL Article ID
    029792201
  • NDL Call No.
    Z54-F482
  • Data Source
    NDL  J-STAGE 
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