Allele specific DNA marker for fusarium resistance gene FocBo1 in Brassica oleracea

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  • Sato Maho
    Laboratory of Plant breeding, Graduate School of Science and Technology, Niigata University Kakegewa Research Center, Sakata Seed Corporation
  • Shimizu Motoki
    Iwate Biotechnology Research Center
  • Shea Daniel J.
    Laboratory of Plant breeding, Graduate School of Science and Technology, Niigata University
  • Hoque Mozammel
    Laboratory of Plant breeding, Graduate School of Science and Technology, Niigata University Faculty of Agriculture, Sylhet Agricultural University
  • Kawanabe Takahiro
    School of Agriculture, Tokai University
  • Miyaji Naomi
    Graduate School of Agricultural Science, Kobe University
  • Fujimoto Ryo
    Graduate School of Agricultural Science, Kobe University
  • Fukai Eigo
    Laboratory of Plant breeding, Graduate School of Science and Technology, Niigata University
  • Okazaki Keiichi
    Laboratory of Plant breeding, Graduate School of Science and Technology, Niigata University

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  • Allele specific DNA marker for fusarium resistance gene <i>FocBo1</i> in <i>Brassica oleracea</i>

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<p>The fusarium yellows resistance (YR) gene FocBo1 was previously identified and the DNA markers were developed to assist the breeding of YR cultivars in Brassica oleracea. However, the further analysis revealed discrepancies between the phenotypes and the genotypes predicted by those DNA markers in cabbage commercial cultivars. Since this discrepancy seemed to be due to unknown susceptible alleles of focbo1, we sequenced the gene in 19 accessions to determine the sequence variations between alleles and found that there were two resistant FocBo1 alleles and six susceptible alleles in the investigated population. The newly designed PCR markers detected three mutations in the susceptible alleles that generate premature termination codons. These were shown to accurately distinguish resistant and susceptible alleles in more than 200 accessions of B. oleracea inbred lines and cultivars. The study revealed that the locus is represented by 37.2% resistant and 62.8% susceptible alleles within seventy-eight commercial cultivars. Structural analysis of the gene revealed that a part of the allelic variation comes from intragenic recombination between alleles. Our results enable a more precise prediction of the phenotype by marker assisted selection, promoting the production of YR cultivars in B. oleracea.</p>

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