Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean
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- Hirata Kaori
- Tohoku Agricultural Research Center, National Agriculture and Food Research Organization (NARO)
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- Takagi Kyoko
- Tohoku Agricultural Research Center NARO
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- Yamada Tetsuya
- Institute of Crop Science, NARO
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- Sayama Takashi
- Western Region Agricultural Research Center, NARO
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- Anai Toyoaki
- Saga University
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- Kikuchi Akio
- Tohoku Agricultural Research Center, National Agriculture and Food Research Organization (NARO)
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- Ishimoto Masao
- Institute of Crop Science, NARO
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Abstract
<p>Food contamination by cadmium (Cd) is a serious threat to human health. Thus, it is imperative to prevent Cd accumulation in staple crops like soybean. The development of low Cd accumulating cultivars is an effective solution. To this end, it is essential to identify the gene(s) controlling seed Cd accumulation. Although Glyma.09G055600 (GmHMA3) seems to be associated with Cd accumulation in soybean, it has not been established if it is responsible for seed Cd accumulation. In the present study, the effect of GmHMA3 on seed Cd accumulation in soybean was validated using three independent GmHMA3 mutants isolated from an ethyl methanesulfonate-induced soybean mutant library. Each of mutant had an amino acid substitution in GmHMA3 and segregating progenies were developed by crossing the original cultivar with each of the three mutants. The relationship between these three mutations and seed Cd accumulation was investigated. While two of them significantly increased seed Cd accumulation corresponding to previous reports of a natural missense mutation in GmHMA3, the other slightly decreased seed Cd accumulation. Overall, these results indicate that GmHMA3 is responsible for seed Cd accumulation in soybean.</p>
Journal
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- Breeding Science
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Breeding Science 69 (2), 345-351, 2019
Japanese Society of Breeding
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Details 詳細情報について
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- CRID
- 1390001288152299520
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- NII Article ID
- 130007679236
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- NII Book ID
- AA11317194
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- ISSN
- 13473735
- 13447610
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- NDL BIB ID
- 029855411
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed